银杏叶提取物（EGb761）对人脂肪间充质干细胞神经向分化的影响

发布时间：2018-03-13 14:14

本文选题：银杏叶提取物　切入点：人脂肪间充质干细胞　出处：《大连医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：观察银杏叶提取物（EGb761）对人脂肪间充质干细胞（hADSCs）增殖及神经向分化的影响。方法：用CCK-8法检测不同浓度EGb761（40mg/L、80mg/L、120mg/L）作用下hADSCs的增殖情况；含有不同浓度EGb761（40mg/L、80mg/L、120mg/L）的联合神经诱导因子（20ng/ml bFGF、20ng/ml EGF、20ng/ml BDNF）对hADSCs神经向诱导分化，观察形态学变化，免疫荧光检测Nestin、NSE、GFAP于3d、7d、10d、14d的表达情况，计数阳性细胞率。结果：EGb761对hADSCs增殖的影响具有时间差异性，1d时无明显差异，3d时显著促进，5d时促增殖趋于平缓，7d时细胞有增殖，但增殖幅度减小；含有不同浓度EGb761的联合诱导因子促进hADSCs的形态学发生神经向分化，与阳性对照组相比各时间点均促进Nestin的表达，，且有浓度依赖趋势；促进NSE的表达，无明显浓度依赖；GFAP的表达与阳性对照无差异。结论：EGb761对hADSCs的增殖有促进作用，3d时促进作用明显；EGb761显著促进hADSCs的神经向分化，不促进向胶质样细胞分化。
[Abstract]:Aim: to observe the effects of Ginkgo biloba extract (EGb761) on the proliferation and neural differentiation of human adipose mesenchymal stem cells (hADSCs). Methods: CCK-8 method was used to detect the proliferation of hADSCs induced by different concentrations of EGb761mg / L 80mg / L (80mg / L), and the expression of Nestin NSEGFAP was detected at 3d7 d 10d ~ 14d by combined nerve inducible factor 20ng / ml bFGF20ngr / ml EGFGF20ngr / ml. The expression of Nestin NSEGFAP was detected by immunofluorescence. The positive cell rate was counted. Results the effects of 1% EGb761 on the proliferation of hADSCs showed no significant difference at 1 day. At 3 days, the proliferation of hADSCs increased significantly at 5 days and tended to be smooth at 7 days, but the range of proliferation decreased. The co-induction factors containing different concentrations of EGb761 promoted the morphological differentiation of hADSCs, and all of them promoted the expression of Nestin in a concentration-dependent manner, and promoted the expression of NSE in a dose-dependent manner, compared with the positive control group. There was no significant difference in the expression of GFAP between the two groups. ConclusionEGB 761 can promote the proliferation of hADSCs. EGb761 can obviously promote the differentiation of hADSCs nerve, but not to glial cells.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R329.2

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