当归饮子加减方对气血两虚模型小鼠迟发型超敏反应的影响

发布时间：2018-03-15 08:46

本文选题：当归饮子　切入点：气血两虚证　出处：《中国中西医结合杂志》2016年03期 　论文类型：期刊论文

【摘要】：目的探讨当归饮子对气血两虚型小鼠迟发型超敏变态反应的影响。方法 SPF级KM小鼠48只,其中40只采用利血平及乙酰苯肼制作动物气血两虚模型,随机分为模型组、氯雷他定组、当归饮子高剂量组、当归饮子中剂量组、当归饮子低剂量组,每组8只;另取8只相同标准小鼠作空白组。当归饮子高、中、低剂量组给予小鼠60、30、15 g/kg当归饮子加减方提取浓缩液灌胃,氯雷他定组给予1.66 mg/kg氯雷他定生理盐水溶液灌胃,模型组和空白组小鼠给予等体积生理盐水灌胃。每日1次,连续给药1周。除空白组外,每只小鼠腹部用1%DNCB溶液均匀涂抹进行致敏,致敏后第5天,将1%DNCB溶液均匀涂抹于小鼠右耳进行攻击,空白组同样涂耳但未致敏,攻击后24 h,测定每组小鼠耳廓肿胀度及抑制率。各组实验动物眼眶取血,采用双抗夹心ELISA法测试血清中Ig E水平。结果与空白组比较,模型组耳肿胀度明显增加,Ig E水平升高(P0.01);与模型组比较,氯雷他定组及当归饮子3个剂量组耳肿胀度降低,Ig E水平降低(P0.05,P0.01),同时当归饮子高、中剂量组耳肿胀度降低优于氯雷他定组(P0.05)。用药各组耳廓肿胀度抑制率从高至低依次为:当归饮子高剂量组67.3%,当归饮子中剂量组56.0%,当归饮子低剂量组48.1%,氯雷他定组47.3%。结论当归饮子方能抑制小鼠耳廓肿胀反应,降低血清中Ig E水平,对气血两虚证小鼠迟发型超敏反应有一定抑制作用。
[Abstract]:Objective to investigate the effect of Radix angelicae sinensis on delayed hypersensitivity in mice with deficiency of qi and blood. Methods Forty SPF grade km mice were randomly divided into two groups: reserpine and acetylphenylhydrazine were used to make animal models of deficiency of qi and blood. Loratadine group, high dose group, medium dose group, low dose group, 8 mice in each group, and 8 mice in the same standard group as blank group. The mice in the low dose group were given 60 mg/kg 30g / kg of Angelica sinensis decoction plus or minus extract, the loratadine group was given 1. 66 mg/kg loratadine saline solution, and the model group and the blank group were given the same volume of normal saline once a day, and the mice in the model group and the blank group were given the same volume of normal saline once a day. Except for blank group, each mouse was sensitized with 1DNCB solution evenly. On the 5th day after sensitization, 1DNCB solution was evenly applied to the right ear of mice for attack. At 24 hours after attack, the auricle swelling and inhibition rate of each group were measured. The blood samples were taken from the orbit of each group and the serum IgE level was measured by double antibody sandwich ELISA method. The results were compared with those in the blank group. Compared with the model group, the ear swelling degree of loratadine group and the three dose groups of Angelica sinensis were lower than that of the model group, and the level of Ig E was lower than that of the model group, and that of Angelica sinensis was higher than that of the model group, while the ear swelling degree of the model group was significantly higher than that of the model group. The inhibition rate of auricle swelling in the middle dose group was higher than that in the loratadine group from high to low: 67.3% in the high dose group, 56.0% in the middle dose group, 48.1% in the low dose group and 48.1% in the loratadine group. Conclusion Danggui decoction can inhibit the swelling reaction of mouse auricle. Decreasing the level of IgE in serum can inhibit the delayed hypersensitivity reaction in mice with deficiency of qi and blood.
【作者单位】：成都中医药大学临床医学院;四川省人民医院四川省医学科学院实验动物研究所;成都中医药大学中医外科教研室;
【基金】：国家自然科学基金资助项目(No.81302982);国家自然科学基金面上项目(No.81573986) 四川省科技厅科技支撑项目(No.2014SZ0090) 四川省教育厅科研基金项目(No.15ZA0085) 成都市科技局科研基金项目(No.2014-HM01-00133-SF) 成都市卫生局科学研究基金项目(No.2014091)
【分类号】：R285.5;R-332

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