枸杞多糖对生精障碍模型小鼠生精能力作用的研究

发布时间：2018-03-16 16:37

本文选题：枸杞子　切入点：枸杞多糖(LBP)　出处：《中华生殖与避孕杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的研究枸杞多糖(LBP)对生精功能和生精干细胞的影响。方法 122只雄性昆明小鼠中随机选取92只,通过35 mg/kg白消安造模,获得生精障碍动物模型,另外30只小鼠为正常对照组。40 d后对造模成功的小鼠随机分为自然恢复组(n=23)和LBP低剂量组(n=16,75 mg/kg)、中剂量组(n=19,150 mg/kg)、高剂量组(n=16,300 mg/kg),连续灌胃给药70 d(小鼠2个生精周期)后进行观察,并与正常对照组进行比较,观察其生理状态和生殖功能变化。结果 LBP在造模动物的外观体征、机能和行为的表现、动物正常发育状态(体质量)、生殖器官组织重量(睾丸、附睾和储精囊、前列腺-会阴复合体)、附睾精子质量、睾丸组织精子生成量、睾丸组织形态学和c-kit表达量、精曲小管直径和空泡化程度、生精上皮厚度和细胞层数、c-kit阳性细胞数、H-score评分、平均吸光度(IA)等系列指标均显示明显高于自然恢复组(P0.05,P0.01),并具有剂量效应关系,但仍未达到正常对照动物的水平。结论 LBP具有明显促进白消安生精障碍模型小鼠的生精能力的作用,可以显著增加生精干细胞、附睾精子质量和睾丸精子生成量。
[Abstract]:Objective to study the effect of Lycium barbarum polysaccharides (LBP) on spermatogenic function and spermatogenic stem cells. The other 30 mice were randomly divided into natural recovery group (n = 23) and LBP low dose group (n = 1675 mg / kg 路kg ~ (-1)), middle dose group (n = 19150 mg / kg) and high dose group (n = 16) (n = 16), 300 mg 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) for 70 d (2 spermatogenic cycles). Results the appearance, function and behavior of LBP in model animals, normal developmental state (body weight, weight of reproductive organs, testis, testis) were observed and compared with those in normal control group. Epididymis and seminal vesicle, prostate-perineum complex, epididymal sperm quality, testicular sperm production, testicular morphology and c-kit expression, seminiferous tubule diameter and degree of vacuolation. The thickness of spermatogenic epithelium and the number of c-kit positive cells in spermatogenic epithelium and the number of c-kit positive cells were significantly higher than those in natural recovery group. Conclusion LBP can promote the spermatogenic ability of mice with spermatogenic disorder, and can significantly increase the spermatogenic stem cells, the sperm quality of epididymis and the quantity of testicular spermatozoa.
【作者单位】：北京协和医学院研究生院;国家卫生计生委科学技术研究所;
【分类号】：R285.5;R-332

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