波形蛋白和胶质纤维酸性蛋白在新生、成年及老年大鼠中枢神经系统表达

发布时间：2018-03-19 09:40

本文选题：波形蛋白　切入点：胶质纤维酸性蛋白　出处：《中南大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的观察Vim和GFAP这两种中间丝蛋白在不同年龄大鼠中枢神经系统的表达变化,从而探讨这两种中间丝蛋白在中枢神经系统发育过程中的可能作用及意义。方法健康SD大鼠24只,雌雄各半,分为新生组(7天龄,n=8)、成年组(5月龄,n=8)、老年组(24月龄,n=8)。用10%水合氯醛溶液(400mg/kg)腹腔注射麻醉。然后按常规依次灌注生理盐水和4%多聚甲醛溶液,稍后取脑组织和脊髓,后固定24h,再经15%、30%蔗糖浸泡,OCT复合物包埋。连续冰冻切片,片厚25μm,然后用Vim和GFAP特异性抗体免疫荧光染色检测这两种蛋白质在海马结构、大脑皮质、脊髓的表达。结果(1)各年龄组大鼠海马结构、大脑皮质、脊髓等部位均有Vim的表达,但在新生鼠表达最强,Vim的表达主要在神经元样细胞。成年和老年鼠Vim的表达主要分布在胞体。(2)各年龄组大鼠海马结构、大脑皮质、脊髓等部位均有GFAP的表达,老年鼠GFAP表达最强。(3)在第三脑室室周,Vim表达在各个年龄阶段没有明显变化,但在新生鼠可见明显的Vim阳性神经元样细胞,成年和老年大鼠间比较GFAP表达没有明显变化。(4)在新生大鼠大脑皮质和海马结构可见Vim和GFAP的共表达,但Vim和GFAP的共表达在第三脑室室周不同发育阶段均可见。结论我们的实验首次系统的报道了Vim和GFAP两种中间丝蛋白在不同年龄大鼠中枢神经系统的表达变化,在大鼠的海马结构、大脑皮质和脊髓等部位Vim的表达随着年龄的增加而下调,GFAP的表达随着年龄的增加而上调；在第三脑室,各年龄组大鼠的Vim表达未见明显变化；Vim主要表达于神经元样细胞,GFAP则主要表达星形胶质细胞,在某些部位这两种蛋白质有共存现象。以上结果提示Vim和GFAP这两种中间丝可能与大鼠中枢神经系统的发育和老化有关,为今后进一步深入研究这两种蛋白质在神经系统发育成熟和老化过程中的作用以及探讨它们在神经损伤、修复、退行性病变和再生的意义提供了一定的实验依据。
[Abstract]:Objective to observe the expression of Vim and GFAP in central nervous system (CNS) of rats of different ages, and to explore the possible role and significance of these two proteins in the development of CNS. Methods Twenty-four healthy SD rats, male and female, were divided into newborn group (n = 7), adult group (n = 8) and aged group (n = 24). 10% mg / kg chloral hydrate solution was given intraperitoneally to anesthetize. Then normal saline and 4% polyformaldehyde solution were infused. The brain tissue and spinal cord were later taken and fixed for 24 hours. The Oct complex was embedded with 15% 30% sucrose. The slices were frozen and the slices were 25 渭 m thick. Then the proteins were detected by Vim and GFAP specific antibody immunofluorescence staining in the hippocampal structure and cerebral cortex. Spinal cord expression. Results 1) the expression of Vim was found in hippocampus, cerebral cortex and spinal cord of rats in all age groups. However, the expression of Vim was mainly in neuron-like cells in newborn rats. The expression of Vim in adult and aged rats was mainly distributed in the cell body. (2) the expression of GFAP was found in hippocampus, cerebral cortex, spinal cord and so on in all age groups of rats. The expression of GFAP in the periventricular area of the third ventricle did not change significantly in the aged rats, but Vim positive neuron-like cells were found in the newborn rats. There was no significant change in the expression of GFAP between adult and aged rats. The expression of Vim and GFAP was observed in the cerebral cortex and hippocampus of neonatal rats, but the co-expression of Vim and GFAP was observed at different stages of periventricular development. Conclusion for the first time, we systematically reported the expression of Vim and GFAP in central nervous system (CNS) of rats of different ages, and the hippocampal structure of rats. The expression of Vim in cerebral cortex and spinal cord was down-regulated with the increase of age, and in the third ventricle, the expression of GFAP was up-regulated with the increase of age. The expression of Vim was mainly expressed in neuron-like cells and astrocytes. These two proteins coexist in some parts of the rat. These results suggest that Vim and GFAP may be involved in the development and aging of the central nervous system in rats. It provides some experimental basis for further study on the role of these two proteins in the development, maturation and aging of the nervous system and their significance in nerve injury, repair, degenerative lesion and regeneration.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329

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