肺炎链球菌不同生长时间自溶素lytA基因表达的差异性分析

发布时间：2018-03-19 21:30

本文选题：肺炎链球菌　切入点：实时荧光定量PCR　出处：《中国病原生物学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨肺炎链球菌lytA基因在细菌生长的不同时间点的表达差异。方法根据GenBank中肺炎链球菌M66株的lytA基因序列(FN 549899.1)设计并合成引物,选取17个生长时间点,分别提取肺炎链球菌的总RNA,采用实时荧光定量PCR技术(QRT-PCR)检测lytA基因在不同生长时间点的相对表达量,利用溶解曲线观察扩增反应特异性,采用双标准曲线法进行数据分析。结果经过内参基因16SRNA校正后,肺炎链球菌lytA基因相对表达量从生长的18h-96h呈现逐渐升高的趋势,96h时达到高峰,与其他时间点比较差异有统计学意义(P0.05)。之后开始下降,144h生长点出现第二次升高趋势。结论不同生长时间点肺炎链球菌lytA基因表达量存在差异,与细菌培养时菌落表现的自溶现象相关,为进一步研究lytA基因与肺炎链球菌自溶现象的相关性奠定了基础。
[Abstract]:Objective to investigate the expression of lytA gene in Streptococcus pneumoniae at different time points. Methods primers were designed and synthesized according to the lytA gene sequence of Streptococcus pneumoniae M66 strain M66 in GenBank, and 17 growth time points were selected. The total RNAs of Streptococcus pneumoniae were extracted, and the relative expression of lytA gene at different growth time points was detected by real-time fluorescence quantitative PCR technique. The specificity of amplification reaction was observed by dissolution curve. Results the relative expression of lytA gene of Streptococcus pneumoniae reached its peak from 18h-96h to 96h after 16SRNA correction. Compared with other time points, the difference was statistically significant (P 0.05). After that, the growth point of Streptococcus pneumoniae increased for a second time. Conclusion there are differences in the expression of lytA gene in Streptococcus pneumoniae at different growth time points. This study was related to the autolysis of bacterial colony, which laid a foundation for the further study of the correlation between lytA gene and the autolytic phenomenon of Streptococcus pneumoniae.
【作者单位】：四川省绵阳市中心医院;大连医科大学临床免疫与微生物教研室;
【基金】：四川省卫计委科技基金资助项目(No.16PJ188)
【分类号】：R378

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