金莲花提取物保护H1N1病毒感染模型小鼠肺脏的实验研究

发布时间：2018-03-19 22:43

  本文选题：流感　切入点：中药　出处：《中国药理学通报》2017年07期 　论文类型：期刊论文

【摘要】：正本课题组前期实验发现流感病毒A/FM/1/47(H1N1)引起小鼠死亡或体重百分数降低,此次实验采用金莲花粗提物灌胃的方法,确定金莲花粗提物的抗病毒作用对小鼠的肺脏的保护作用。1材料与方法1.1病毒株亚洲甲型鼠肺适应株A/FM/1/47(H1N1),中国预防医学科学院病毒学研究所提供。血凝滴度1∶512,Reed Muench法测定对小鼠的半数致死量(LD_(50))10~(-3.68)。1.2主要试剂及仪器金莲花粗提物(由本课题组其他成员提取获得),JJ200型电子天平,常熟双杰测试仪器厂。
[Abstract]:The original preliminary experiments found that influenza virus A/FM/1/47 (H1N1) mice caused by death or weight percentage decreased and the method used in this experiment Jinlianhua crude extract gavage, determine the antiviral effect of Jinlianhua lung extracts on mice and the protective effect of.1 materials and methods of 1.1 influenza virus strains of Asian mouse lung adapted strains (A/FM/1/47 H1N1), China prevention Virology Research Academy of Medical Sciences. The hemagglutination titer of 1: 512, the determination of the lethal dose of half of mouse Reed Muench (LD_ (50)) 10~ (-3.68).1.2 main reagents and instruments Jinlianhua extracts (other members of the group by the extraction, JJ200 electronic) balance, Changshu Shuangjie testing instrument factory.

【作者单位】： 北京中医药大学;
【基金】：国家自然科学基金资助项目(No 81274044)
【分类号】：R285.5;R-332
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本文编号：1636345