痛泻要方对IBS模型血清内源性物质代谢干预的实验研究

发布时间：2018-03-21 12:03

本文选题：痛泻要方　切入点：肠易激综合征　出处：《中国中药杂志》2017年05期 　论文类型：期刊论文

【摘要】：通过代谢组学评价痛泻要方对肠易激综合征(irritable bowel syndrome,IBS)大鼠模型血清内源性物质代谢干预效应,寻找潜在的生物标志物并分析其代谢途径,探讨痛泻要方的作用机制及病证模型的证候本质。将40只Wistar大鼠复制为IBS模型,随机分为模型对照组和痛泻要方给药组4组,另设空白对照组10只。痛泻要方(低、中、高)组分别灌胃剂量为0.203,0.406,0.812 g·m L~(-1)的痛泻要方,空白对照组及模型对照组给予等体积的生理盐水,每日1次,连续2周。各组大鼠于灌胃第0,15天采集血清,经处理后供UPLC-Q-TOF-MS进行代谢组学分析。鉴定出8个潜在生物标志物,分析出8条主要代谢通路,其与IBS疾病的神经递质代谢、炎性免疫、脑神经功能及能量代谢等有关,痛泻要方对IBS疾病的作用机制可能涉及血清素突触和色氨酸代谢、半胱氨酸和甲硫氨酸代谢、甘油磷脂代谢、烟酸和烟酰胺代谢等过程,其可能是IBS模型肝旺脾虚证的生物学基础。
[Abstract]:To evaluate the effect of Tongxieyao recipe on the metabolism of endogenous substances in serum of irritable bowel syndrome (IBS) rats, to search for potential biomarkers and to analyze its metabolic pathway. To explore the mechanism of Tongxieyao recipe and the syndromes essence of the disease syndrome model, 40 Wistar rats were duplicated into IBS model, which were randomly divided into four groups: model control group and Tongxieyiefang administration group, and another 10 rats in blank control group. The rats in the control group were given the same volume of normal saline once a day for 2 weeks respectively. The serum samples were collected from rats in each group on the 15th day after oral administration of Tongxie recipe (0.203 ~ 0.406g 路m ~ (-1)), the blank control group and the model control group were given the same volume of normal saline once a day for 2 weeks. Eight potential biomarkers were identified and 8 major metabolic pathways were identified, which were related to neurotransmitter metabolism, inflammatory immunity, brain nerve function and energy metabolism of IBS disease. The mechanism of Tongxie decoction on IBS disease may involve serotonin synaptic and tryptophan metabolism, cysteine and methionine metabolism, glycerol phospholipid metabolism, nicotinic acid and nicotinamide metabolism, etc. It may be the biological basis of liver and spleen deficiency syndrome in IBS model.
【作者单位】：黑龙江中医药大学;哈尔滨市食品药品监督管理局;黑龙江中医药大学省部共建北药基础与应用研究重点实验室;黑龙江省中药及天然药物药效物质基础研究重点实验室;黑龙江中医药大学附属第一医院;黑龙江中医药大学附属第二医院;
【基金】：国家自然科学基金面上项目(81573870) 中国博士后科学基金第8批特别资助项目(2015T80376);中国博士后科学基金项目(2013M531079) 黑龙江省自然科学基金面上项目(H2015020) 黑龙江中医药大学优秀创新人才支持计划项目(051217)
【分类号】：R285.5;R-332

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