TREM-1与铜绿假单胞菌感染和定植的相关性研究

发布时间：2018-03-22 10:39

本文选题：铜绿假单胞菌　切入点：髓样细胞触发受体　出处：《泸州医学院》2012年硕士论文　论文类型：学位论文

【摘要】：目的：通过研究髓样细胞触发受体-1(triggering receptor expressed onmyeloid cells-1，TREM-1) mRNA及血清sTREM-1在铜绿假单胞菌(Pseudomonas aeruginosa, PA)呼吸系统感染和定植不同状态下的表达水平的变化，探讨TREM-1与铜绿假单胞菌肺炎及呼吸道定植的相关性，从而为在病原学培养中病原菌为定植菌抑或致病菌的判断提供理论及实验依据、更好的早期感染预警、反应临床预后判断及指导抗生素的使用。方法：1.将SD大鼠按随机原则分成肺部感染组、口咽部定植组及对照组。环甲膜穿刺注入铜绿假单胞菌（PA）菌液建立大鼠肺炎模型，定植组则由大鼠口、鼻腔内注入PA菌液，并将环甲膜穿刺注入无菌生理盐水作为对照，并于建模后第3、9、24小时分别随机抽取并予以处死后收集标本。2.收集各组不同时间点处死的大鼠血液，以ELISA法检测血清中sTREM-1、CRP和PCT的水平；采用PCR法检测肺组织中TREM-1mRNA的表达水平；肺组织病理切片比较炎症的范围及程度，同时行肺组织匀浆菌落计数，定植组加行咽拭子细菌培养。3.应用SPSS软件对数据进行统计分析，数据采用X+S表示。组间比较采用单因素方差分析，sTREM-1与CRP、PCT线性相关采用Pearson相关分析。结果：1.感染组各时间点大鼠肺组织中TREM-1mRNA的表达水平较定植组及对照组均有明显的增高，差异有统计学意义（P＜0.05），其中细菌接种3小时后TREM-1mRNA的表达就有升高，9小时达到高峰，24小时开始回落。定植组与对照组TREM-1mRNA表达比较没有显著差异。2.感染组各时间点大鼠血清中sTREM-1、CRP和PCT的水平较定植组及对照组均有明显的增高，差异有统计学意义（P＜0.05）。其中sTREM-1、CRP及PCT水平在3小时开始上升，9小时升高到峰值，24小时后下降。相关性分析中sTREM-1和CRP、PCT均成正相关，相关系数分别为0.86和0.75。3.感染组大鼠肺组织病理改变在9小时最为严重，，炎症范围广，程度重，3、24小时与之相比均较轻，而定植组和对照组的肺泡及肺间质结构基本正常，无明显出血、炎细胞浸润；感染组肺组织均培养出铜绿假单胞菌，肺组织匀浆菌落计数均105CFU/g，定植组和对照组肺组织均未培养出细菌，定植组咽拭子细菌培养均有呼吸道正常菌群及铜绿假单胞菌生长。结论：1.铜绿假单胞菌肺部感染其肺组织TREM-1mRNA和血清sTREM-1表达明显增高，且与咽部定植和对照组存在明显差异。TREM-1及sTREM-1的检测有助于铜绿假单胞菌感染与定植的鉴别。 2.铜绿假单胞菌肺部感染肺组织TREM-1mRNA和血清sTREM-1表达水平与肺部炎症的程度有关，TREM-1的检测对感染性疾病预后判断有指导意义。3.铜绿假单胞菌肺炎血清sTREM-1和炎症指标CRP，PCT水平变化呈高度正相关，sTREM-1在铜绿假单胞菌感染中有重要意义。
[Abstract]:Objective: to study the changes of the expression levels of mRNA and serum sTREM-1 in Pseudomonas aeruginosa (PAA) in different respiratory infection and colonization conditions by using the myeloid cell trigger receptor expressed onmyeloid cells-1tREM-1 (TREM-1) mRNA, a myeloid cell trigger receptor, and to investigate the changes of the expression level of sTREM-1 in different respiratory system infection and colonization of Pseudomonas aeruginosa (PAA). To explore the correlation between TREM-1 and pneumonia and respiratory tract colonization of Pseudomonas aeruginosa, so as to provide a theoretical and experimental basis for the identification of pathogenic bacteria as colonizing bacteria or pathogenic bacteria in etiological culture, and to provide a better early warning of infection. Methods Sprague-Dawley rats were randomly divided into three groups: pulmonary infection group, oropharyngeal colonization group and control group. In the colonization group, PA bacteria were injected into the nasal cavity of the rats, and the cyclidine puncture was injected into the sterile saline as the control group. The blood samples of rats were collected at different time points to detect the levels of serum sTREM-1CRP and PCT by ELISA method, and the expression of TREM-1mRNA in lung tissue by PCR method. The range and degree of inflammation were compared by pathological section of lung tissue, and the colony count of lung tissue homogenate was carried out, and the bacterial culture of pharynx swab was added to the colonization group. The data were analyzed by SPSS software. The data were expressed by X S. The linear correlation between TREM-1 and CRPP-PCT was analyzed by univariate ANOVA and Pearson correlation analysis. Results: 1. The expression of TREM-1mRNA in lung tissue of infected rats was significantly higher than that of colonized group and control group at each time point. The difference was statistically significant (P < 0.05), in which the expression of TREM-1mRNA increased at 3 hours after inoculation and reached its peak at 9 hours and began to decrease at 24 hours. There was no significant difference in TREM-1mRNA expression between the colonized group and the control group. 2.The expression of TREM-1mRNA in the infected rats at different time points was not significantly different from that in the control group. The levels of serum sTREM-1CRP and PCT were significantly higher than those in the colonized group and the control group. The difference was significant (P < 0.05). The levels of sTREM-1CRP and PCT began to rise at 3 hours and then increased to a peak at 9 hours and then decreased after 24 hours. There was a positive correlation between sTREM-1 and sTREM-1 in correlation analysis. The correlation coefficients were 0.86 and 0.75.3, respectively. The pathological changes of lung tissue in the infected group were the most serious at 9 hours, with a wide range of inflammation. The lung interstitial structure of the colonized group and the control group was basically normal, compared with the control group for 24 hours. Pseudomonas aeruginosa was cultured in the lung tissue of the infected group, the colony count of the homogenate of the lung tissue was 105 CFU / g, and the bacteria were not cultured in the lung tissue of the colonization group and the control group. In the colonized group, the normal bacterial flora and the growth of Pseudomonas aeruginosa were found in the respiratory tract. Conclusion: 1. The expression of TREM-1mRNA and serum sTREM-1 in pulmonary infection of Pseudomonas aeruginosa was significantly increased. The detection of TREM-1 and sTREM-1 was helpful for the identification of Pseudomonas aeruginosa infection and colonization. 2. The expression of TREM-1mRNA and serum sTREM-1 in pulmonary tissue of Pseudomonas aeruginosa pulmonary infection is related to the degree of pulmonary inflammation. The detection of TREM-1 has guiding significance in judging the prognosis of infectious disease .3.The serum sTREM-1 and the inflammatory index of Pseudomonas aeruginosa pneumonia. The level change of TREM-1 is highly positively correlated with the infection of Pseudomonas aeruginosa (Pseudomonas aeruginosa).
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R378

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