电针对MCAO模型大鼠海马区内源性神经干细胞表达的影响

发布时间：2018-03-23 04:36

本文选题：脑梗死　切入点：内源性神经干细胞　出处：《中国中西医结合杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的观察电针对大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)后缺血再灌注(ischemia-reperfusion,I/R)不同时间点大鼠海马区内源性神经干细胞(endogenous neural stem cells,e NSCs)表达的影响,探讨电针对急性脑梗死(acute cerebral infarction,ACI)远隔损害的可能机制。方法雄性SPF级SD大鼠用线栓法制作MCAO模型,采用随机数字表法分为假手术组、脑梗死组、电针组,每组30只;假手术组仅做手术创伤,脑梗死组仅作MCAO缺血再灌注处理;电针组选取"百会"和"大椎"行电针治疗,每天1次,每次30 min;治疗后第1、7、14天分别评定神经功能缺损程度,同时在各组随机选取6只大鼠处死,分离出缺血侧海马组织,采用免疫荧光法检测海马组织e NSCs增殖与分化表达。结果 (1)大鼠神经功能缺损评分方面:与假手术组比较,脑梗死组在第1、7、14天神经功能缺损评分增高(P0.05);与脑梗死组比较,电针组在第1、7、14天神经功能缺损评分降低(P0.05)。(2)Brd U阳性细胞表达:与假手术比较,脑梗死组缺血侧海马CA1、CA3、DG处Brd U阳性细胞数在第1、7、14天均增多(P0.05);与脑梗死组比较,电针组Brd U阳性细胞数均增多(P0.05)。(3)Nestin阳性细胞表达:与假手术组比较,脑梗死组海马齿状回Nestin阳性细胞数在第1、7、14天均增多(P0.05);与脑梗死组比较,电针组Nestin阳性表达增加,但仅第7天差异明显(P0.05)。(4)DCX阳性细胞表达:与假手术组比较,脑梗死组第1、7天DCX阳性细胞增多(P0.05);与脑梗死组比较,电针组第7、14天DCX阳性细胞增多(P0.05)。(5)Neu N阳性细胞表达:与假手术组比较,脑梗死组海马齿状回Neu N阳性细胞表达在第1、7、14天均增加,但仅第14天时差异明显(P0.05);与脑梗死组比较,电针组Neu N阳性细胞表达增多,但仅第1、14天时差异明显(P0.05)。(6)GFAP阳性细胞表达:与假手术组比较,脑梗死组缺血侧海马齿状回GFAP阳性细胞表达在第1、7、14天均明显增多(P0.05);与脑梗死组比较,电针组GFAP阳性细胞表达增多不显著,仅第14天时差异明显(P0.05)。结论大鼠脑梗死后海马区存在e NSCs增殖与分化,电针治疗可促进脑梗死后神经缺损功能恢复,其机制可能与电针促进脑梗死海马区e NSCs增殖与分化,抑制e NSCs过度分化为星形胶质细胞,及早长时程促进e NSCs分化为神经元,促进神经再生等机制密切相关。
[Abstract]:Objective to observe the effect of electroacupuncture on the expression of endogenous neural stem cells (NSCs) in hippocampus of rats after ischemia reperfusion I / R at different time points after middle cerebral artery occlusion (MCAO). To explore the possible mechanism of electroacupuncture (EA) on the distant damage of acute cerebral infarction (ACI). Methods male SD rats of SPF grade were divided into three groups: sham operation group, cerebral infarction group and electroacupuncture group with 30 rats in each group with 30 rats in each group. The sham operation group was treated only with surgical trauma, the cerebral infarction group was treated with MCAO ischemia-reperfusion only, and the electroacupuncture group was treated with "Baihui" and "Dazhui" for 30 minutes, once a day. At the same time, 6 rats were killed at random in each group, the hippocampal tissues of ischemic side were isolated, and the proliferation and differentiation of e NSCs in hippocampus were detected by immunofluorescence method. Results 1) the neurological deficit score of rats was compared with that of sham operation group. Compared with the cerebral infarction group, the nerve function defect score of the electroacupuncture group decreased the expression of P0.05 + BrdU on the 1st day of the 7th day: compared with the sham-operated group, and compared with the sham-operation group, the neurologic deficit score of the electroacupuncture group decreased the expression of P0.05 + BrdU on the 14th day after the first day of cerebral infarction, and compared with the sham operation, there was no significant difference between the two groups. In cerebral infarction group, the number of Brd U positive cells in hippocampal CA1 + CA3 + DG was increased at 714 days after cerebral infarction, and compared with cerebral infarction group, the number of Brd U positive cells in electroacupuncture group was higher than that in cerebral infarction group: compared with sham operation group, the number of Brd U positive cells in electroacupuncture group was higher than that in sham operation group. The number of Nestin positive cells in the dentate gyrus of the cerebral infarction group increased on the 1st day (P 0.05), compared with the cerebral infarction group, the positive expression of Nestin increased in the electroacupuncture group, but on the 7th day there was a significant difference: compared with the sham operation group, the positive expression of Nestin in the electroacupuncture group was higher than that in the sham operation group. Compared with the cerebral infarction group, the expression of DCX positive cells in the electroacupuncture group was higher than that in the sham operation group on day 714, and the expression of the DCX positive cells in the electroacupuncture group was higher than that in the sham operation group, compared with the sham operation group, the expression of DCX positive cells in the electroacupuncture group was higher than that in the sham operation group. The expression of Neu N in dentate gyrus of cerebral infarction group was increased on the 1st day, but on the 14th day, the difference was significant (P 0.05), compared with that in the cerebral infarction group, the expression of Neu N positive cells in electroacupuncture group was higher than that in the cerebral infarction group. However, the expression of GFAP in dentate gyrus of ischemic side in cerebral infarction group was significantly higher than that in sham-operated group on the 1st day after 714 days, compared with that in the cerebral infarction group, and was significantly higher than that in the cerebral infarction group on day 1, 714, and compared with that in the cerebral infarction group, but the expression of GFAP-positive cells in the dentate gyrus of the ischemic side in the cerebral infarction group was significantly higher than that in the sham-operated group. The expression of GFAP positive cells in the electroacupuncture group was not significantly increased, but the difference was significant at the 14th day. Conclusion there is e NSCs proliferation and differentiation in hippocampus after cerebral infarction in rats. Electroacupuncture treatment can promote the recovery of nerve defect function after cerebral infarction. The mechanism may be related to the mechanism of electroacupuncture promoting the proliferation and differentiation of e NSCs in hippocampus of cerebral infarction, inhibiting the overexdifferentiation of e NSCs into astrocytes, promoting the differentiation of e NSCs into neurons early and long term, and promoting nerve regeneration.
【作者单位】：广东省佛山市中医院神经内科;南方医科大学南方医院神经科;
【基金】：国家自然科学基金资助项目(No.81473470,81072947) 广东省自然科学基金重点项目(No.8152800007000001,2014A030311033) 佛山市院市合作项目(No.2014HT10004)
【分类号】：R245;R-332

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