伊潘立酮联合阿立哌唑对慢性铝暴露痴呆模型大鼠海马神经元及BDNF和NGF表达的影响

发布时间：2018-03-23 05:35

本文选题：伊潘立酮　切入点：阿立哌唑　出处：《毒理学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的旨在探讨伊潘立酮联合阿立哌唑对慢性铝暴露痴呆模型大鼠海马神经元及BDNF和NGF表达的影响。方法选择6周龄清洁级SD雄性大鼠建立慢性铝暴露痴呆模型,分为模型组、伊潘立酮组、阿立哌唑组和联合组,每组各20只,并同时设健康大鼠20只为对照组。伊潘立酮组大鼠接受0.05 g/ml伊潘立酮0.25 g/kg灌胃,阿立哌唑组大鼠接受0.05 g/ml阿立哌唑0.25 g/kg灌胃,联合组大鼠接受0.05 g/ml伊潘立酮0.25 g/kg和0.05 g/ml阿立哌唑0.25 g/kg灌胃,对照组大鼠和模型组大鼠均接受同等剂量生理盐水灌胃。各组大鼠均进行学习记忆测试和旷场实验测试。Annexin V/FITC法测定大鼠海马组织神经元凋亡率、RT-PCR法检测大鼠海马组织BDNF及NGF mRNA表达、Western blot法检测大鼠海马组织BDNF及NGF蛋白表达。结果对照组和联合组大鼠电击次数显著低于模型组大鼠(P0.05),而水平穿越格数和竖立次数显著高于模型组大鼠(P0.05)。联合组大鼠电击次数低于伊潘立酮组和阿立哌唑组大鼠,水平穿越格数和竖立次数高于伊潘立酮组和阿立哌唑组大鼠,但组间差异无统计学意义(P0.05)。对照组、联合组、伊潘立酮组和阿立哌唑组大鼠海马组织神经凋亡率均显著低于模型组大鼠(P0.05)。对照组、联合组、伊潘立酮组和阿立哌唑组大鼠海马组织BDNF及NGF mRNA、蛋白表达均显著高于模型组大鼠(P0.01)。结论伊潘立酮联合阿立哌唑能显著抑制慢性铝暴露痴呆模型大鼠海马神经元凋亡,上调BDNF及NGF表达。
[Abstract]:Objective to investigate the effects of ipristone combined with aripiprazole on the expression of BDNF and NGF in hippocampal neurons of rats with chronic aluminum exposure dementia. Methods A 6-week-old male SD rat model of chronic aluminum exposure dementia was established and divided into two groups. There were 20 rats in each group, 20 in each group, and 20 healthy rats in each group. The rats in the control group were perfused with 0. #number0# g/kg of Ipperidone, and 0. 05 g/ml of aripiprazole with 0. 25 g/kg in the group of aripiprazole, and the rats in the control group were given 0. 05 g/ml of aripiprazole 0. 25 g/kg by gavage. The rats in the combined group were given intragastric administration of #number0# g/kg and 0.05 g/ml aripiprazole for 0. #number0# g/kg and 0. 05 g/ml, respectively. Rats in the control group and the model group were given the same dose of normal saline. The learning and memory test and open field test. Annexin V/FITC method were used to detect the apoptosis rate of hippocampal neurons in rats. RT-PCR method was used to detect the hippocampal neuron apoptosis in rats. The expression of BDNF and NGF protein in hippocampus of rats was detected by BDNF and NGF mRNA. Results the number of electric shock in the control group and the combined group was significantly lower than that in the model group, while the number of horizontal traverses and the number of erections were significantly higher than that in the model group. The number of electric shocks in the combined group was lower than that in the Ipperidone group and aripiprazole group. The number of horizontal traversals and the number of erections were higher than those in the Ipperidone group and aripiprazole group, but there was no significant difference between the two groups (P 0.05). The rate of neuronal apoptosis in hippocampal tissue of Ipperidone group and aripiprazole group was significantly lower than that of model group (P 0.05). The expression of BDNF and NGF mRNAs in hippocampal tissue of the rats in the Ipperidone group and aripiprazole group were significantly higher than those in the model group (P 0.01). Conclusion the apoptosis of hippocampal neurons in the model rats exposed to chronic aluminum exposure can be significantly inhibited by the combination of Ipperidone and aripiprazole. The expression of BDNF and NGF was up-regulated.
【作者单位】：郑州市第八人民医院药械科;河南省中医院中心实验室;武汉疗养院;
【分类号】：R-332;R965

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