结核分枝杆菌感染对巨噬细胞内胆固醇酯的影响

发布时间：2018-03-24 19:40

本文选题：结核分枝杆菌　切入点：巨噬细胞　出处：《中国病原生物学杂志》2017年03期

【摘要】：目的探讨结核分枝杆菌感染对巨噬细胞内胆固醇酯的影响。方法将小鼠RAW264.7细胞分为空白对照组和结核分枝杆菌感染(细菌数与细胞数之比分别为5∶1、10∶1、20∶1)实验组,孵育1、3、5、7d后采用油红O染色观察细胞内脂滴的变化,利用酶法测定细胞内总胆固醇、游离胆固醇和胆固醇酯相对含量的变化。结果细菌数与细胞数之比为20∶1孵育1d,油红O染色细胞内可见大量脂滴,胆固醇酯相对含量为(65.91±2.45)%,孵育7d,大量细胞出现裂解,脂滴溢出。细胞内胆固醇酯的含量随时间和菌量的增加而升高。结论结核分枝杆菌感染可以导致小鼠巨噬细胞内胆固醇酯含量增加,且与时间和菌量成正比。
[Abstract]:Objective to investigate the effect of Mycobacterium tuberculosis infection on cholesterol ester in macrophages. Methods RAW264.7 cells in mice were divided into blank control group and Mycobacterium tuberculosis infection group (the ratio of bacterial number to cell number was 5: 1 10: 1: 20: 1). Oil red O staining was used to observe the changes of lipid droplets and total cholesterol in the cells was determined by enzymatic method after incubation for 7 days. Results when the ratio of bacterial number to cell number was 1 day at 20:1, a large number of lipid droplets could be found in the cells stained with oil red O, and the relative content of cholesterol ester was 65.91 卤2.45%. After incubation for 7 days, a large number of cells were lysed. Lipid spill. The content of cholesterol ester in cells increased with the increase of time and bacteria. Conclusion Mycobacterium tuberculosis infection can lead to the increase of cholesterol ester content in mouse macrophages, which is directly proportional to the time and the amount of bacteria.
【作者单位】：潍坊医学院医学检验学系;潍坊医学院临床学院病原生物学教研室;
【基金】：国家自然科学基金项目(No.81470001,81170080) 山东省自然科学基金资助项目(No.ZR2016HM09)
【分类号】：R378.911

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