姜黄素对PD模型细胞活性氧簇水平及沉默信息调控因子3表达的影响

发布时间：2018-03-26 07:23

本文选题：帕金森病　切入点：姜黄素　出处：《山东医药》2017年40期

【摘要】：目的探讨姜黄素对鱼藤酮诱导的帕金森病(PD)模型SH-SY5Y细胞活性氧簇(ROS)水平及沉默信息调控因子3(Sirt3)表达的影响,为PD的临床治疗提供依据。方法将体外培养的SH-SY5Y细胞分为姜黄素1组、姜黄素2组、姜黄素3组、姜黄素4组、模型组和对照组。姜黄素1、2、3、4组先分别加入0.5、1.0、5.0、10.0μmol/L的姜黄素培养4 h,然后加入0.1μmol/L鱼藤酮共同培养24 h;模型组直接加入0.1μmol/L鱼藤酮培养24 h;对照组培养基内不加任何药物培养24 h。采用DCFH-DA染色法检测各组ROS水平,RT-PCR法测定Sirt3 mRNA相对表达量,Western blotting法测定Sirt3蛋白相对表达量。结果 ROS水平:模型组高于对照组(P0.05),姜黄素2组姜黄素1组模型组(P均0.05),但姜黄素3组、姜黄素4组、模型组两两比较P均0.05。Sirt3 mRNA和蛋白相对表达量:模型组低于对照组(P均0.05),姜黄素2组姜黄素1组模型组(P均0.05),但姜黄素3组、姜黄素4组、模型组两两比较P均0.05。结论姜黄素可降低SH-SY5Y细胞ROS水平,上调Sirt3表达;此作用姜黄素浓度为1.0μmol/L时最为明显。
[Abstract]:Objective to investigate the effect of curcumin on the expression of rotenone-induced rotenone-induced reactive oxygen species (Ros) in SH-SY5Y cells and the expression of silencing signal regulator 3- Sirt3. Methods SH-SY5Y cells cultured in vitro were divided into three groups: curcumin group 1, curcumin group 2, curcumin group 3, curcumin group 4. The model group and the control group were cultured for 4 h respectively with 0. 5 渭 mol/L rotenone for 0. 5 渭 mol/L and then 0. 1 渭 mol/L rotenone for 24 h respectively. The model group was cultured directly with 0. 1 渭 mol/L rotenone for 24 h; the control medium was not cultured with any drugs. The relative expression of Sirt3 mRNA in each group was detected by DCFH-DA staining method and the relative expression of Sirt3 mRNA by RT-PCR. Results ROS level of model group was higher than that of control group (P 0.05), curcumin group 2 was curcumin group 1 model group. P, 0.05, but curcumin group 3, The relative expression of 0.05.Sirt3 mRNA and protein in the model group was lower than that in the control group (P < 0.05), curcumin group 1 model group (P 0.05), curcumin group 3, curcumin group 4, but curcumin group 3 and curcumin group 4. Conclusion curcumin can decrease the level of ROS and up-regulate the expression of Sirt3 in SH-SY5Y cells, especially when the concentration of curcumin is 1.0 渭 mol/L.
【作者单位】：郑州大学第二附属医院;
【基金】：河南省科学技术厅基础与前沿技术研究计划项目(14230-0410461)
【分类号】：R285.5;R-332

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