基于细胞调控因子探讨血尿胶囊对急性肾盂肾炎模型大鼠的作用及机制

发布时间：2018-03-26 07:27

本文选题：血尿胶囊　切入点：急性肾盂肾炎　出处：《中成药》2017年08期

【摘要】：目的通过观察血尿胶囊对急性肾盂肾炎模型大鼠脏器指数、细胞因子以及肾脏组织的影响,探讨血尿胶囊治疗急性肾盂肾炎的作用机制。方法采用"半结扎"输尿管,膀胱注射大肠杆菌方法制备急性肾盂肾炎大鼠模型,治疗组于造模后第3天起每天给药1次,正常组及模型组给予等量生理盐水,连续10 d,第13天处死动物,检测脏器指数,测定血清中血肌酐、IL-1α(白细胞介素-1α)、IL-1β、IL-6、IL-10、CXCL-2(趋化因子-2)的含有量,对右侧结扎肾脏进行细菌培养并行病理组织学检查。结果大鼠造模后,左右肾比值、肾脏指数增大(P0.01),膀胱指数有所升高(P0.05);造模大鼠IL-1α、IL-1β、IL-6、IL-10、血肌酐、尿肌酐、CXCL-2含有量均显著升高(P0.05,P0.01);模型组大鼠右侧结扎肾脏细菌培养阳性率为80%;给药10 d后,血尿胶囊高、中剂量组左右肾比值、肾脏指数下降(P0.01),接近于空白组,膀胱指数变化不明显;血尿胶囊高、中剂量组IL-1α、IL-6、IL-10含有量均明显下降(P0.01),血尿胶囊高剂量组IL-1β含有量下降明显(P0.01),血尿胶囊高、中、低剂量组尿肌酐、CXCL-2均有所降低(P0.05,P0.01),血肌酐含有量并无明显变化(P0.05);血尿胶囊高剂量组细菌培养阳性率为20%,中剂量组为50%,低剂量组为60%;病理组织检查结果显示血尿胶囊能够显著降低急性肾盂肾炎大鼠肾脏感染、减轻炎症反应。结论血尿胶囊能够改善肾功能,修复肾小球基底膜,降低尿肌酐含有量,下调趋化因子水平,减轻急性肾盂肾炎模型大鼠的肾脏炎症反应,达到治疗急性肾盂肾炎的目的。
[Abstract]:Objective to investigate the mechanism of Xueniao capsule in treating acute pyelonephritis by observing the effects of Xueniao capsule on organ index, cytokines and renal tissue in rats with acute pyelonephritis. The model of acute pyelonephritis was made by intravesical injection of Escherichia coli. The rats in the treatment group were given the same amount of normal saline once a day from the 3rd day after the model, and the normal group and the model group were given the same amount of normal saline for 10 days. The animals were killed on the 13th day and the visceral index was measured. Serum levels of serum creatinine interleukin-1 伪 (IL-1 伪) were determined, and the ratio of left and right kidneys was determined by bacterial culture and histopathological examination after the right ligation of the kidney, and the contents of CXCL-2 (chemokine 2) were measured. The positive rate of IL-1 伪 IL-1 尾 IL-6 IL-10, serum creatinine, urinary creatinine CXCL-2 was significantly increased in the model rats, and the positive rate of the right side ligation of kidney bacteria was 80 in the model group, 10 days after administration, the blood and urine capsules were high. In the middle dose group, the ratio of left and right kidney, the decrease of renal index and the decrease of renal index were close to the blank group, but the bladder index did not change obviously, the content of IL-1 伪 IL-6 IL-10 in the middle dose group decreased significantly, and the content of IL-1 尾 decreased significantly in the high dose group of Xueniao capsule, and the content of IL-1 尾 in the high dose group was significantly lower than that in the control group, and the content of IL-10 in Xueliao capsule was higher than that in the control group. Urinary creatinine CXCL-2 was decreased in low dose group, and serum creatinine content was not significantly changed. The positive rate of bacterial culture was 20 in the high dose group, 50 in the middle dose group and 60 in the low dose group. Pathological examination showed that the blood creatinine content of Xueniao capsule was 60%, and that of the high dose group was 20%, while that of the middle dose group was 50%, and that of the low dose group was 60%. Can significantly reduce renal infection in rats with acute pyelonephritis. Conclusion Xueliao capsule can improve renal function, repair glomerular basement membrane, reduce urinary creatinine content, down-regulate chemokine level, and alleviate renal inflammation in rats with acute pyelonephritis. To achieve the treatment of acute pyelonephritis.
【作者单位】：山西省中医药研究院;山西中医学院;山西振东制药股份有限公司;
【基金】：山西省科技攻关项目(山西省中药现代化关键技术研究振东专项)(2014ZD0203)
【分类号】：R285.5;R-332

【相似文献】