BCG初免-单次重组腺病毒Ad5-CEAB加强免疫策略的免疫效应研究（英文）

发布时间：2018-03-26 07:34

  本文选题：结核分枝杆菌　切入点：初免-加强　出处：《中国人兽共患病学报》2017年06期

【摘要】：目的为了评价BCG初免-单次重组腺病毒Ad5-CEAB加强的免疫策略诱导小鼠产生的免疫效应。方法在前期工作的基础上,制备并纯化获得了高滴度的重组腺病毒Ad5-CEAB,然后以小鼠为动物模型,采用BCG初免-Ad5-CEAB加强的异源初免-加强的免疫策略对动物进行免疫,通过测定小鼠淋巴细胞抗原刺激液中IL-12和IFN-γ的含量,测定小鼠血清IgG抗体含量以及小鼠肺盥洗液中sIgA的浓度来评价BCG初免-单次重组腺病毒Ad5-CEAB加强免疫策略的免疫效应。结果 BCG初免-Ad5-CEAB加强免疫后,经抗原刺激后的小鼠淋巴细胞会分泌高水平的IL-12和IFN-γ,这两种细胞因子的浓度均显著高于对照组。BCG初免-Ad5-CEAB加强后,还可以有效的刺激小鼠分泌IgG抗体,血清IgG抗体浓度显著高于对照组。另外,小鼠肺盥洗液中sIgA的浓度也显著高于对照组。结论采用BCG初免-单次Ad5-CEAB加强的异源初免-加强的免疫策略能够有效的刺激小鼠产生较强的免疫应答反应。
[Abstract]:Objective to evaluate the immune effect induced by BCG primary immune-single recombinant adenovirus Ad5-CEAB enhanced immunization strategy in mice. Methods on the basis of previous work, The recombinant adenovirus Ad5-CEAB with high titer was prepared and purified. Then mice were used as an animal model to immunize animals with the strategy of allogeneic immune-enhancement enhanced by BCG primary immune-Ad5-CEAB. The contents of IL-12 and IFN- 纬 in mouse lymphocyte antigen stimulating fluid were determined. The level of serum IgG antibody and the concentration of sIgA in mouse lung lavatory fluid were determined to evaluate the immune effect of BCG primary immune-single recombinant adenovirus Ad5-CEAB. The lymphocytes stimulated by antigen could secrete high levels of IL-12 and IFN- 纬. The concentrations of these two cytokines were significantly higher than those of the control group. The concentration of the two cytokines was significantly higher than that of the control group. After immunized with -Ad5-CEAB, they could effectively stimulate the secretion of IgG antibody in mice. The concentration of serum IgG antibody was significantly higher than that of the control group. The concentration of sIgA in mouse lung lavatory solution was also significantly higher than that in control group. Conclusion the strategy of BCG first immune-single time Ad5-CEAB enhanced xenogeneic immune-enhancement can effectively stimulate the mice to produce a strong immune response.
【作者单位】： 宁夏大学西部特色生物资源保护与利用教育部重点实验室;
【基金】：Supported by grant from the National Natural Science Foundation of China(Grant Nos.31572494,31160515,31560678)~~
【分类号】：R392
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本文编号：1666980