乙型肝炎病毒相关性肾炎裸鼠模型的建立

发布时间：2018-03-27 21:37

本文选题：BALB/c裸小鼠　切入点：乙型肝炎病毒　出处：《华中科技大学》2012年硕士论文

【摘要】：目的：建立乙型肝炎病毒相关性肾炎裸鼠模型。方法：BALB/c裸小鼠20只，模型组、对照组各10只，观察一周小鼠尿常规正常后，向模型组裸小鼠腹腔内注射含HBV-DNA"g107"ml的人血清0.75ml／只，连续4周，每周2次，对照组注射等量0.9%生理盐水。在动物注射血清7天后，每三天查尿一次，进行尿蛋白半定量（试纸条法），直至尿蛋白＞++后，每2周进行尿蛋白半定量一次。对照组处理方法同模型组。至第5周开始，每2周上述两组动物各处死2只，进行尿蛋白半定量、取肾组织并行光镜病理、透射电镜及免疫荧光检查。第9周处死所有鼠并做上述检查。结果：模型组裸小鼠4~9周时尿蛋白持续阳性(++~+++)；肾组织光镜病理学检查呈轻、中、重度系膜增生性肾小球肾炎改变，部分肾小管间质炎性浸润；免疫荧光发现HBsAg阳性，荧光强度为+~+++；透射电镜检查：在系膜区可见电子致密物沉积。对照组的光镜检查肾小球及肾间质均未见明显病变，，免疫荧光阴性，电镜亦无异常发现。结论：模型组裸小鼠尿蛋白阳性，光镜下发现系膜呈不同程度增生；HBsAg间接免疫荧光+~+++；透射电镜下发现系膜区有免疫复合物沉积，基本符合乙肝相关性肾炎的诊断标准，所以，该乙肝肾动物模型的建立是成功的。
[Abstract]:Objective: to establish a nude mouse model of hepatitis B virus associated glomerulonephritis (HBV). Methods Twenty BALB / C nude mice, 10 in model group and 10 in control group, were injected intraperitoneally with human serum 0.75ml/ containing HBV-DNA "g107" ml for 4 weeks, twice a week, after observing the normal urine routine of mice in the model group for one week. In the control group, 0.9% saline was injected into the control group. After 7 days of serum injection, urine was checked once every 3 days. The control group was treated with the same method as the model group. From the fifth week, 2 animals were killed in each of the two groups every 2 weeks. All the rats were killed at the 9th week. Results: in the model group, urine protein was continuously positive in nude mice at 4 ~ 9 weeks. Light microscopic pathological examination of renal tissue showed slight, moderate and severe Mesangial proliferative glomerulonephritis, partial tubulointerstitial inflammatory infiltration, and HBsAg positive by immunofluorescence. The fluorescence intensity was ~; transmission electron microscopy showed that electron dense material was deposited in the Mesangial region. There was no obvious pathological changes in glomeruli and renal interstitium in the control group, immunofluorescence was negative, and there was no abnormal finding in electron microscope. Conclusion: in the model group, urine protein was positive in nude mice, the Mesangium was found to be proliferated with different degrees of HBsAg indirect immunofluorescence under light microscope, and the deposition of immune complex in Mesangial region was found under transmission electron microscope, which basically met the diagnostic criteria of hepatitis B associated glomerulonephritis. Therefore, the establishment of the hepatitis B kidney animal model is successful.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332;R512.62;R692.3

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