消退素D1对非压迫性腰椎间盘突出症模型大鼠背根神经节炎症的作用

发布时间：2018-03-28 12:58

本文选题：消退素D　切入点：腰椎间盘突出症　出处：《中国疼痛医学杂志》2016年05期

【摘要】：目的:研究消退素D1(Resolvin D1,Rv D1)对非压迫性腰椎间盘突出症模型大鼠背根神经节(dorsal root ganglion,DRG)炎症的影响及内在机制。方法:选取30只雄性大鼠建立非压迫性腰椎间盘突出症模型。随机分为假手术组、模型组、Rv D1组(100 ng)。术后连续3天模型组和Rv D1组分别鞘内注射10μl磷酸盐缓冲液、Rv D1。于术前1天及术后连续7天检测各组大鼠术侧50%缩足阈值(50%paw withdrawal threshold,PWT)。术后第7天,酶联免疫吸附测定术侧L5 DRG中TNF-α和IL-1β的蛋白表达量,免疫组化法检测DRG中p-ERK及NF-κB/p65的阳性表达。结果:术后各时间点,模型组大鼠50%PWT较假手术组明显降低(P0.01);TNF-α和IL-1β表达明显升高(P0.01);p-ERK、NF-κB/p65蛋白水平明显升高(P0.01)。术后第2~7天,Rv D1组与模型组相比,50%PWT显著升高(P0.05);TNF-α和IL-1β的蛋白表达明显降低(P0.01);p-ERK及NF-κB/p65蛋白水平明显降低(P0.01)。结论:Rv D1促进背根神经节炎症的消退,可能与调节炎症介质TNF-α和IL-1β的表达及ERK和NF-κB/p65通路活性有关。
[Abstract]:Objective : To study the effects and internal mechanisms of melatonin D1 ( Rv D1 ) on the inflammation of dorsal root ganglion ( DRG ) in rats with non - compression lumbar disc herniation . Methods : Thirty male rats were selected to establish a model of non - compression lumbar disc herniation . Ten 渭l of phosphate buffer solution and Rv D1 were injected into the sheath of the 3 - day and Rv - D1 groups after operation , and the 50 % paw withdrawal threshold was detected in each group on the first day before operation and 7 days after operation . Results : The expression of TNF - 伪 and IL - 1尾 in DRG was significantly decreased ( P0.01 ) . The expression of TNF - 伪 and IL - 1尾 in DRG increased significantly ( P0.01 ) , and the expression of TNF - 伪 and IL - 1尾 in DRG was significantly decreased ( P0.01 ) .

【作者单位】：山东大学附属省立医院疼痛科;
【基金】：国家自然科学基金(81271232) 国家临床重点专科建设项目经费资助山东省科技发展计划(2014GSF118130)
【分类号】：R285.5;R-332

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