不同血瘀证亚型目征特点与血小板活化及炎症损伤关系的实验研究

发布时间：2018-03-29 05:33

本文选题：血瘀证目征　切入点：球结膜微循环　出处：《南昌大学》2012年硕士论文

【摘要】：目的：研究五种不同血瘀证亚型目征改变的特点及其与血小板活化功能及炎症损伤的关系。方法：将60只健康日本大耳兔按随机分组法分为6组，分别为气虚血瘀组、气滞血瘀组、寒凝血瘀组、热毒血瘀组、外伤血瘀组、正常对照组进行造模。比较各组血瘀证目征改变及积分的变化、同时观测各组球结膜微循环、血清血小板膜糖蛋白P-选择素(CD62P)、肿瘤坏死因子-α（TNF-a）水平的变化。结果：(1)造模前各组血瘀证目征积分无显著差异(P0.05)；(2)造模后血瘀证目征积分各模型组与正常对照组及各自造模前相比均有统计学意义(P0.05)；(3)治疗后，血瘀证目征积分血瘀证各亚型组与各自造模后相比均有统计学意义(P0.05)。(4)造模前各组血清CD62P，TNF-a的水平无显著差异(P0.05)；(5)造模后血清CD62P，TNF-a水平各模型组与正常对照组及各自造模前相比，具有显著性差异(P0.01)；(6)治疗后，，血清CD62P，TNF-a水平血瘀证各亚型组与各自造模后相比，具有显著性差异(P0.01)；(7)造模后气滞血瘀组CD62P明显高于其他亚型组，热毒血瘀组TNF-a明显高于其他亚型组，差异均有统计学意义（P0.05）。结论：血瘀证目征积分是诊断血瘀证的有效方法，但目征总积分并不能用于区别血瘀证不同亚型，需要借助血瘀证目征具体形态变化及各亚型不同的病理表现及球结膜微循环改变进行综合判断。血瘀证各亚型之间有明确的血瘀证共性，但又各具特点。
[Abstract]:Objective: to study the characteristics of five subtypes of blood stasis syndrome and their relationship with platelet activation function and inflammatory injury. Methods: sixty healthy Japanese rabbits were randomly divided into 6 groups: Qi deficiency and blood stasis group, Qi stagnation and blood stasis group, cold coagulation and blood stasis group, heat toxin and blood stasis group, and traumatic blood stasis group. The changes of ocular sign and integral of blood stasis syndrome in each group were compared, and the changes of bulbar conjunctival microcirculation, serum platelet membrane glycoprotein (P-selectin) CD62PU and tumor necrosis factor- 伪 (TNF-a) were observed in normal control group. Results (1) there was no significant difference in the score of blood stasis syndromes in each group before and after modeling (P 0.05). There was statistical significance after treatment in each model group compared with the normal control group and the control group. There was no significant difference in serum CD62PtTNF-a levels before and after model making between each model group and each model group. The serum CD62PU TNF-a level in each model group was higher than that in the normal control group and before the model making, and there was no significant difference between each model group and the normal control group before and before the establishment of the model, and there was no significant difference in the serum CD62PU TNF-a level between the model group and the normal control group. After treatment, the levels of serum CD62PnTF-a in blood stasis syndrome group were significantly different from those after model making. (P 0.01) the CD62P of Qi stagnation and blood stasis group was significantly higher than that of other subtype group, and the TNF-a of heat toxin blood stasis group was significantly higher than that of other subtype group. The difference was statistically significant (P 0.05). Conclusion: the integral of eye sign of blood stasis syndrome is an effective method for diagnosis of blood stasis syndrome, but the total integral of eye sign can not be used to distinguish different subtypes of blood stasis syndrome. It is necessary to make a comprehensive judgment with the specific morphological changes of the eye signs of blood stasis syndrome, the different pathological manifestations of each subtype and the changes of bulbar conjunctiva microcirculation. There are definite similarities of blood stasis syndrome among the subtypes of blood stasis syndrome, but each has its own characteristics.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

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