空肠弯曲菌AhpC蛋白B细胞抗原表位预测及抗原性分析

发布时间：2018-03-29 10:24

本文选题：空肠弯曲菌　切入点：表位　出处：《中国人兽共患病学报》2017年10期

【摘要】：目的利用生物信息学预测分析空肠弯曲菌AhpC的跨膜结构、信号肽及B细胞抗原表位,并分析空肠弯曲菌AhpC优势B细胞抗原表位的抗原性,为后续疫苗研究提供依据。方法使用TMHMM Server V2.0、SignalP 4.1、IEDB等生物信息学分析软件对空肠弯曲菌AhpC进行蛋白跨膜结构预测、信号肽及线性B细胞抗原表位进行预测分析。分别以原核表达的空肠弯曲菌AhpC蛋白和化学合成的表位肽为抗原,空肠弯曲菌AhpC抗体为一抗,通过ELISA及Dot blot对优势线性B细胞抗原表位的抗原性进行分析及筛选。结果生物信息学预测结果表明AhpC定位于空肠弯曲菌外膜,无跨膜结构,无信号肽,同时该蛋白中存在7个的B细胞线性表位。经Dot blot和ELISA检测发现其中AhpC4-16表位具有较强的抗原性,为优势表位。结论空肠弯曲菌AhpC保守存在于细菌表面,存在1个优势的B细胞线性抗原表位(AhpC4-16),可用于后续的疫苗开发研究。
[Abstract]:Objective to predict and analyze the transmembrane structure, signal peptide and B cell antigen epitopes of Campylobacter jejuni AhpC by bioinformatics, and to analyze the antigenicity of dominant B cell epitopes of Campylobacter jejuni AhpC. Methods TMHMM Server V2.0 Signal P 4.1 IEDB and other bioinformatics software were used to predict the protein transmembrane structure of Campylobacter jejuni AhpC. The signal peptide and linear B cell antigen epitope were predicted and analyzed. The prokaryotic expressed AhpC protein and the chemically synthesized epitope peptide were used as antigens, respectively, and the AhpC antibody against Campylobacter jejuni was a primary antibody against Campylobacter jejuni. The antigenicity of dominant linear B cell epitopes was analyzed and screened by ELISA and Dot blot. Results the bioinformatics prediction results showed that AhpC was located in the outer membrane of Campylobacter jejuni with no transmembrane structure and no signal peptide. At the same time, there were 7 B cell linear epitopes in the protein. The AhpC4-16 epitopes were found to have strong antigenicity by Dot blot and ELISA. Conclusion the AhpC of Campylobacter jejuni is conserved on the surface of bacteria. There is a dominant B cell epitope AhpC4-16, which can be used for further vaccine development.
【作者单位】：金华职业技术学院医学院分子生物学实验室;金华职业技术学院人畜共患病研究所;
【基金】：浙江省科技计划项目(No.2016C33229);浙江省科技计划项目(No.2016C37093) 金华市科技研究计划项目(No.2016-3-002)联合资助~~
【分类号】：R392

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