四对结核分枝杆菌毒素-抗毒素系统基因功能的初步研究

发布时间：2018-03-29 13:34

本文选题：结核分枝杆菌　切入点：毒素-抗毒素系统　出处：《中国人兽共患病学报》2017年05期

【摘要】：目的探讨结核分枝杆菌毒素-抗毒素(toxin-antitoxin,TA)系统中的4对基因的功能,为研究结核分枝杆菌的传播机制提供基础科学数据。方法选取结核分枝杆菌TA系统VapBC家族中4对基因,包括VapC 4个毒素基因(Rv1720c、Rv2103c、Rv2494和Rv3408)和VapB4个抗毒素基因(Rv1721c、Rv2104c、Rv2493、Rv3407),在大肠杆菌和耻垢分枝杆菌中分别构建严谨型阿拉伯糖诱导质粒体系及乙酰胺诱导穿梭质粒体系观察VapC对细菌生长的抑制作用,及其对应的VapB解除抑制作用。结果在大肠杆菌和耻垢分枝杆菌的实验结果一致,Rv2103c具有一定抑制作用和Rv2104c具有消除抑制作用,其余3对毒素-抗毒素基因未见到明显的对细菌生长的抑制和消除抑制的作用。结论成功构建了VapBC家族在大肠杆菌及耻垢分枝杆菌中的表达体系,并发现了一对TA系统基因对细菌生长的抑制和消除抑制的作用。
[Abstract]:Objective to investigate the function of four pairs of genes in the Mycobacterium tuberculosis toxin-antitoxin (TAA) system, and to provide basic scientific data for the study of the transmission mechanism of Mycobacterium tuberculosis. Methods four pairs of genes were selected from the VapBC family of Mycobacterium tuberculosis TA system. Four VapC toxin genes Rv1720c#en0# Rv2103cU Rv2494 and Rv3408) and VapB4 antitoxin gene Rv1721cnrv2104c1 Rv2493407 were used to construct rigorous arabinose inducing plasmids and acetamide induced shuttle plasmids in Escherichia coli and Mycobacterium smeareus respectively to observe the inhibitory effect of VapC on bacterial growth. Results in the experiments of Escherichia coli and Mycobacterium smearus, Rv2103c had a certain inhibitory effect and Rv2104c had an anti-inhibition effect, and the inhibitory effect of Rv2103c was similar to that of Rv2103c. Conclusion the expression system of VapBC family in Escherichia coli and Mycobacterium smegmatis was successfully constructed. The inhibition and elimination of bacterial growth by a pair of TA system genes were also found.
【作者单位】：温州医科大学检验医学院生命科学学院浙江省模式生物技术与应用重点实验室;首都医科大学附属北京胸科医院国家结核病临床实验室;北京师范大学生命科学学院教育部细胞增殖及调控生物学重点实验室;
【基金】：国家自然科学基金(81273144) 北京市自然科学基金B类重点项目(KZ201510025024)~~
【分类号】：R378.911

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