Toll样受体5对间充质干细胞生物学特性的影响

发布时间：2018-03-31 00:41

本文选题：Toll样受体　切入点：间充质干细胞　出处：《军事医学》2017年07期

【摘要】：目的探讨Toll样受体5(TLR5)对间充质干细胞(MSC)生物学特性的影响,以期探究TLR5在MSC与肿瘤关系中的作用。方法构建过表达TLR5的慢病毒载体并用相关病毒感染MSC,通过荧光表达量、RT-q PCR和Western印迹验证TLR5在MSC中的过表达情况,通过MSC-TLR5增殖、表面抗原和细胞分化来验证TLR5过表达对MSC生物学性状影响,通过检测CBLB502激活TLR5后白细胞介素6(IL-6)和IL-8的表达水平验证TLR5生物学功能。结果 TLR5慢病毒感染MSC后,mRNA水平和蛋白水平检测TLR5表达量均显著升高,MSC-TLR5增殖、免疫表型和分化能力未发生改变,同时,CBLB502激活TLR5后,IL-6和IL-8表达量显著升高(P0.001)。结论携带TLR5的慢病毒感染MSC后不影响MSC生物学功能,TLR5具有功能活性且可激活下游信号通路发挥免疫调节作用。
[Abstract]:Objective to investigate the effects of Toll like receptor 5 (TLR5) on the biological characteristics of mesenchymal stem cells (MSCs). Methods Lentivirus vector expressing TLR5 was constructed and infected with TLR5. The overexpression of TLR5 in MSC was verified by fluorescent expression of RT-q PCR and Western blotting, and proliferation of TLR5 by MSC-TLR5. Surface antigen and cell differentiation were used to verify the effect of TLR5 overexpression on the biological characteristics of MSC. The biological function of TLR5 was verified by detecting the expression level of interleukin-6 (IL-6) and IL-8 after TLR5 activated by CBLB502. Results the expression of TLR5 in MSC infected with TLR5 lentivirus significantly increased the proliferation of MSC-TLR5. The immunophenotype and differentiation ability did not change. At the same time, the expression of IL-6 and IL-8 increased significantly after activation of TLR5 by CBLB502. Conclusion Lentivirus carrying TLR5 does not affect the biological function of MSC after MSC infection and can activate downstream signaling pathway to play an immunomodulatory role.
【作者单位】：云南农业大学动物科学技术学院;北京放射与辐射医学研究所;
【基金】：国家自然科学基金资助项目(81270894)
【分类号】：R329.2

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