猫视神经连续切片的计算机三维重建

发布时间：2018-03-31 00:27

本文选题：视神经　切入点：三维重建　出处：《解剖学研究》2010年06期

【摘要】：目的制备猫视神经的连续切片,选择合适的染色方法 ,寻找合适的配准方法 ,经图像采集及初步处理后利用计算机的三维重建技术,获得可视化的猫视神经三维结构。方法利用石蜡切片技术的制备猫视神经连续切片,采用特殊的神经三色染色法进行切片染色。利用数码显微镜及Motic Images Advanced 3.0和Motic Images Assembly 1.0软件对连续的猫视神经石蜡切片进行拍照,获得猫视神经连续切片的原始图像,将原始图像在GIMP软件中经过调整亮度对比度、初步图像配准、提取神经束膜图像信息后在Amira软件下,经过计算机在手工配准的基础上进一步自动配准,把二维的切片图像重建成三维的可视化图像。结果实验所得经三色染色的连续切片的原始图像上神经束膜能较好的与周围的组织区分;利用Amira三维重建软件,重建得到猫视神经三维结构图像,重建后的猫视神经结构三维图像可进行任意的切割、旋转、回复、拍照等操作。结论实验证明在不利用标志线的条件下,通过制备视神经连续切片,经过染色拍照,手工图像配准后利用计算机自动配准技术对视神经进行三维重建是完全可行的。
[Abstract]:Objective to prepare the serial sections of the optic nerve of the cat, to select the appropriate staining method and to find the appropriate registration method, and to obtain the visual three-dimensional structure of the optic nerve of the cat by using the three-dimensional reconstruction technique of the computer after the image acquisition and preliminary processing.Methods Cat optic nerve serial sections were prepared by paraffin technique and stained by special nerve trichrome staining.After the initial image registration and the extraction of the neural bundle membrane image information, the two-dimensional slice image is reconstructed into a three-dimensional visual image by computer automatic registration on the basis of manual registration under the Amira software.Results the nerve trachea on the original images of the serial slices stained by three colors could be distinguished from the surrounding tissues, the three-dimensional reconstruction software of Amira was used to reconstruct the three-dimensional structure images of the optic nerve of the cat.The reconstructed 3D image of optic nerve structure of cat can be cut, rotated, restored, photographed and so on.Conclusion it is proved by experiments that it is feasible to reconstruct the optic nerve by computer automatic registration technique by making continuous sections of the optic nerve without using the mark line and taking pictures by staining and taking pictures by hand.
【作者单位】：复旦大学上海医学院解剖与组织胚胎学系;复旦大学附属眼耳鼻喉医院;
【基金】：国家973项目(2005CB724306) 国家自然科学基金(30971536)
【分类号】：R77

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