致病菌与糖的特异性识别及其分析应用研究

发布时间：2018-03-31 14:55

本文选题：糖-凝集素相互作用　切入点：多效价相互作用　出处：《分析测试学报》2017年06期

【摘要】：致病菌往往通过凝集素-糖特异性识别来实现对宿主细胞的粘附,进而感染宿主组织,引起病变。因此,研究致病菌与糖的特异性识别有利于进一步了解感染性疾病的致病机制,为致病菌的特异性检测和感染性疾病的治疗提供新的策略。该文总结了致病菌-糖特异性识别的相关机制机理;介绍了目前主要的研究方法和技术,特别评述了荧光光谱、表面等离子体共振、电化学阻抗谱及石英晶体微天平等技术在该研究中的应用现状,并对这4种技术与微流控芯片平台的结合进行了探讨;针对致病菌检测特异性差、耐药性严重等难题,重点综述了致病菌-糖的特异性识别在细菌分离、富集、检测、鉴别、生物膜抑制及抗菌糖类药物筛选方面的应用。最后对致病菌-糖特异性识别基础和应用研究进行了展望。
[Abstract]:Pathogenic bacteria often achieve adhesion to host cells by lectin-sugar specific recognition, and then infect host tissues, causing pathological changes.Therefore, the study of the specific recognition of pathogenic bacteria and sugar is helpful to further understand the pathogenic mechanism of infectious diseases, and to provide a new strategy for the specific detection of pathogenic bacteria and the treatment of infectious diseases.In this paper, the mechanism of glycose-specific recognition is summarized, and the main research methods and techniques are introduced, especially the fluorescence spectrum, surface plasmon resonance (SPR), surface plasmon resonance (SPR), surface plasmon resonance (SPR).The application status of electrochemical impedance spectroscopy and quartz crystal microbalance in this research is discussed, and the combination of these four technologies with microfluidic chip platform is discussed.The application of specific recognition of pathogenic bacteria-sugar in isolation, enrichment, detection, identification, biofilm inhibition and screening of antimicrobial carbohydrate drugs was reviewed.Finally, the basis and application of sugar specific recognition of pathogenic bacteria were prospected.
【作者单位】：重庆大学化学化工学院;重庆大学新型微纳器件与系统技术国防重点学科实验室;重庆大学微纳系统及新材料技术国际研发中心;重庆大学光电工程学院微系统研究中心;
【基金】：国家自然科学基金项目(21375156) 国家高技术研究发展计划(863)项目(2015AA021104) 重庆市前沿研究重点项目(cstc2015jcyj BX0010) 重庆市科技惠民项目(cstc2015shmszx00014)
【分类号】：O657;R378

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