针刺对血管性痴呆模型大鼠大脑皮质神经元凋亡及p-JNK表达的影响

发布时间：2018-04-02 01:33

本文选题：血管性痴呆　切入点：针刺　出处：《中医杂志》2017年11期

【摘要】：目的探讨针刺治疗血管性痴呆的可能作用机制。方法 24只大鼠随机分为假手术组、模型组、针刺组和非穴组,每组6只。除假手术组外其余各组应用双侧颈总动脉永久性结扎方法制备血管性痴呆大鼠模型。应用Nissl染色观测其皮质神经元形态学改变,应用TUNEL法观察皮质神经元凋亡情况,应用免疫荧光技术观察磷酸化c-Jun氨基末端激酶(p-JNK)表达。结果与假手术组比较,模型组大鼠大脑皮质细胞排列散乱,核固缩、深染,且大脑皮质凋亡神经元增多,p-JNK阳性细胞数增多(P0.05);与模型组比较,针刺组大鼠皮质神经元形态改善,凋亡神经元较模型组显著减少,p-JNK表达减少(P0.05);而非穴组无明显改善。结论针刺可抑制血管性痴呆大鼠大脑皮质p-JNK表达,减少大脑皮质凋亡神经元,可能是其治疗血管性痴呆的机制之一。
[Abstract]:Objective to explore the possible mechanism of acupuncture in the treatment of vascular dementia.Methods Twenty-four rats were randomly divided into sham-operation group, model group, acupuncture group and non-point group with 6 rats in each group.Vascular dementia model was established by permanent ligation of bilateral common carotid artery in all groups except sham operation group.Morphological changes of cortical neurons were observed by Nissl staining, apoptosis of cortical neurons was observed by TUNEL method, and p-JNKs expression of phosphorylated c-Jun amino-terminal kinases was observed by immunofluorescence technique.Results compared with the sham-operated group, the cerebral cortex cells in the model group were disorganized, nuclear pyknosis, deep staining, and the number of p-JNK positive cells increased in the cerebral cortex of the model group, and the morphology of the cortex neurons in the acupuncture group was improved compared with the model group.Compared with the model group, the apoptotic neurons decreased the expression of p-JNK significantly, but the non-acupoint group did not significantly improve the expression of p-JNK.Conclusion Acupuncture can inhibit the expression of p-JNK in cerebral cortex and decrease the apoptotic neurons in cerebral cortex of vascular dementia rats, which may be one of the mechanisms of acupuncture in the treatment of vascular dementia.
【作者单位】：首都医科大学附属北京中医医院;
【基金】：北京市科技新星计划(2014B063)
【分类号】：R245;R-332

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