微创压力超负荷小鼠心衰模型的建立

发布时间：2018-04-02 02:10

本文选题：压力超负荷　切入点：心衰模型　出处：《中国胸心血管外科临床杂志》2016年11期

【摘要】：目的通过胸骨上窝途径微创建立小鼠心衰模型。方法选用雄性昆明种系小鼠,经胸骨上窝利用钽夹部分夹闭主动脉弓,通过测量心脏体重比(HW/BW)、心脏超声以及组织学检查观察该方法创建小鼠心衰模型可行性。结果部分夹闭主动脉弓10周,HW/BW较假手术组明显增加(P0.01);左室舒张期末直径(LVEDD)显著增大(P0.01);左室后壁舒张期末厚度(LVPWD)明显变薄(P0.01);射血分数(EF)以及短轴缩短率(FS)显著变小(P0.01);夹闭部分主动脉弓10周后其血清氨基末端B型利钠肽前体(NT-pro BNP)较假手术组明显增加(P0.01)。组织学观察左室部分心肌纤维排列紊乱、断裂,不同程度的结构破坏,心肌细胞呈不同程度变性及肥大,部分心肌细胞呈局灶性或片状坏死。结论经胸骨上窝途径部分夹闭主动脉弓建立小鼠心衰模型方法简单易行、微创、可靠。
[Abstract]:Objective to establish mouse model of heart failure on the sternum nest way minimally invasive. Methods: male Kunming mice, the suprasternal fossa using tantalum clamp closed aortic arch, by measuring the ratio of heart weight to body weight (HW/BW), cardiac ultrasound and histological examination to observe the method of creating the model of heart failure in mice. Results the feasibility partially occluded aortic arch 10 week, HW/BW compared with the sham group was significantly increased (P0.01); left ventricular end diastolic diameter (LVEDD) increased significantly (P0.01); left ventricular end diastolic wall thickness was significantly thinner after (LVPWD) (P0.01); ejection fraction (EF) and fractional shortening (FS) was significantly smaller (P0.01) the clipping part of the aortic arch; after 10 weeks the serum amino terminal B type natriuretic peptide (NT-pro BNP) compared with the sham group was significantly increased (P0.01). Histological observation of left ventricular myocardial fibers arranged in disorder, fracture, structural damage degree of myocardial cells showed different degrees of change Some of the myocardial cells showed focal or flaky necrosis. Conclusion it is simple, minimally invasive and reliable to establish a mouse heart failure model by partially clamping the aortic arch through the sternum approach.

【作者单位】：上海交通大学医学院附属新华医院心胸外科;吉林大学第一医院心脏外科;宁夏自治区人民医院西北民族大学附属第一临床学院心胸外科;
【基金】：吉林省科技发展计划资助项目(20080730)~~
【分类号】：R-332;R541.6

【相似文献】