弓形虫Toxofilin蛋白基因克隆和生物信息学分析

发布时间：2018-04-02 05:11

本文选题：弓形虫　切入点：Toxofilin基因　出处：《中国病原生物学杂志》2017年08期

【摘要】：目的运用生物信息学方法预测分析弓形虫Toxofilin基因编码蛋白的功能域及三级结构。方法 PCR扩增Toxofilin全长基因序列,利用SignaIP和TMHMM在线网站预测分析其编码蛋白的信号肽、跨膜区;利用DNAStar软件分析Toxofilin蛋白的亲水性、抗原指数;联合运用ExPASY和PRODATA分析Toxofilin蛋白的功能域及三级结构。结果 PCR扩增Toxofilin基因大小为738bp。DNAstar分析其编码蛋白Toxofilin序列存在典型信号肽,但不存在跨膜区,在其N端存在较高的亲水性基团和抗原指数。ExPASY分析Toxofilin蛋白存在酰基化、磷酸化蛋白激酶位点。结论 Toxofilin蛋白含有信号肽和磷酸化位点等功能域,抗原指数,为验证Toxofilin功能奠定了理论基础。
[Abstract]:Objective to predict and analyze the functional domain and tertiary structure of Toxofilin gene encoding protein of Toxoplasma gondii by bioinformatics. Methods the full-length Toxofilin gene sequence was amplified by PCR, and its signal peptide and transmembrane region were predicted and analyzed by SignaIP and TMHMM online website. DNAStar software was used to analyze the hydrophilicity and antigen index of Toxofilin protein, and ExPASY and PRODATA were used to analyze the functional domain and tertiary structure of Toxofilin protein. Results the size of Toxofilin gene amplified by PCR was a typical signal peptide in 738bp.DNAstar analysis of Toxofilin sequence of its encoded protein. However, there was no transmembrane region. There were high hydrophilic groups and antigen index. ExPASY analysis showed that Toxofilin protein had acylation and phosphorylated protein kinase site. Conclusion Toxofilin protein contains functional domains such as signal peptide and phosphorylation site, and antigen index. It lays a theoretical foundation for verifying the function of Toxofilin.
【作者单位】：山东省医学科学院山东省寄生虫病防治研究所;山东大学齐鲁医院(青岛);
【基金】：国家自然基金青年项目(No.31502057,81501770) 山东省医学科学院医药卫生科技创新工程项目
【分类号】：R382.5

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