手针针刺治疗佐剂性关节炎疼痛模型效应平台的建立及影响因素

发布时间：2018-04-03 09:48

本文选题：足三里穴　切入点：手针针刺　出处：《辽宁中医杂志》2016年12期

【摘要】：目的:建立可靠、稳定的手针针刺镇痛效应平台。方法:选择佐剂性关节炎疼痛模型为研究平台,先后以2种不同浓度的造模药物以及3种手针针刺刺激参数开展了研究。结果:采用低浓度佐剂(含结核杆菌浓度为1 mg/m L)造模后24 h大鼠致炎侧足底痛阈明显下降,并且可持续到观察周期结束(造模后21 d);高浓度佐剂(10 mg/m L)造模后24 h大鼠致炎侧足底痛阈亦明显下降,但在造模后容易出现足底溃疡等不良反应,影响指标的监测。针刺单侧足三里穴,未见明显的镇痛效应;针刺双侧足三里穴,留针30 min(其间每隔5 min行捻转手法,平补平泻,180次/min,持续2min)可有效抑制佐剂性关节炎大鼠的疼痛症状。结论:采用低浓度佐剂造模,手针针刺双侧足三里穴可有效建立针刺治疗佐剂性关节炎疼痛模型效应平台。在此效应平台的建立过程中,存在多种影响因素,主要包括实验动物、造模药物浓度、针刺干预参数、效应指标等因素,在具体研究过程中,应当综合考虑这些因素。
[Abstract]:Objective: to establish a reliable and stable platform for acupuncture analgesia.Methods: the pain model of adjuvant arthritis was selected as the research platform. Two kinds of different concentrations of model drugs and three kinds of acupuncture stimulation parameters were used.Results: the pain threshold in the plantar of the inflammatory side of rats was significantly decreased 24 h after the use of low concentration adjuvant (including 1 mg/m / L of Mycobacterium tuberculosis).The pain threshold of the inflammatory side of the plantar was also decreased 24 hours after the establishment of the model (21 days after modeling; 10 mg/m / L of high concentration adjuvant), but the adverse reactions such as plantar ulcer were easy to occur after modeling, which affected the monitoring of the indexes.Acupuncture at Zusanli point on one side had no obvious analgesic effect, and the pain symptoms of adjuvant arthritis rats could be effectively inhibited by acupuncture at bilateral Zusanli acupoint for 30 minutes (twirling every 5 min during twirling manipulation, pingBuping Xielong 180 times / min, lasting 2 min).Conclusion: using low concentration adjuvant to make model and acupuncture at bilateral Zusanli point can effectively establish the model platform of acupuncture for adjuvant arthritis pain.In the process of establishing this effect platform, there are many influencing factors, including experimental animals, drug concentration, acupuncture intervention parameters, effect index and so on. These factors should be considered comprehensively in the concrete research process.
【作者单位】：天津市南开医院;浙江省中医院;天津中医药大学实验针灸学研究中心;湖南中医药高等专科学校;
【分类号】：R684.3

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