激素联合去卵巢诱导大鼠股骨骨质疏松复合模型的建立

发布时间：2018-04-03 14:06

本文选题：骨质疏松　切入点：地塞米松　出处：《中国组织工程研究》2017年24期

【摘要】：背景:使用激素的绝经后女性人群在临床上并不少见,由于具备雌激素水平不足及使用激素两个因素,故骨量流失的速度更快,更容易发生脆性骨折。目前对于研究与该人群相匹配的复合模型,即激素联合去卵巢诱导的骨质疏松模型的水平不够深入,相关的分子机制尚未完全阐明。目的:构建激素联合去卵巢股骨骨质疏松大鼠模型,并探讨复合模型的分子机制。方法:将3月龄雌性SD大鼠随机分为空白对照组、假手术组及模型组,各10只。空白对照组不造模,假手术组切开腹部剪取少量脂肪组织,模型组从腹部切除双侧卵巢同时连续4周注射地塞米松。结果与结论:造模后4周,与空白对照组及假手术组相比,模型组离体股骨骨密度、骨小梁数量、相对骨体积显著降低,骨小梁间距显著提高,且骨小梁间距增宽和结构破坏,骨皮质变薄,骨皮质厚度降低,骨组织Runx2 m RNA表达显著下调,Ⅰ型胶原α1、过氧化物酶体增殖物激活受体γmR NA表达明显升高,组织蛋白酶k mR NA表达呈上升趋势,但差异无显著性意义。提示激素联合去卵巢可快速诱导大鼠股骨骨质疏松,其机制可能与下调Runx2,上调Ⅰ型胶原α1、过氧化物酶体增殖物激活受体γ的mR NA表达有关。
[Abstract]:Background: postmenopausal women who use hormones are not uncommon in clinical practice. Due to the lack of estrogen and the use of hormones, bone mass loss is faster and brittle fractures are more likely to occur.At present, the level of study on the combination of hormone and ovariectomized osteoporosis model is not deep enough, and the molecular mechanism has not been fully elucidated.Aim: to establish a rat model of estrogen combined with ovariectomized femur osteoporosis and to explore the molecular mechanism of the compound model.Methods: 3 months old female SD rats were randomly divided into control group, sham operation group and model group with 10 rats each.No model was made in the blank control group. In the sham operation group, a small amount of adipose tissue was cut off from the abdomen, and dexamethasone was injected into the model group for 4 weeks after bilateral ovaries were excised from the abdomen.The bone cortex thinned, the thickness of bone decreased, the expression of Runx2 m RNA decreased significantly, the expression of type I collagen 伪 1, peroxisome proliferator activated receptor 纬 -mRNA increased, and the expression of cathepsin k mrna increased.But there was no significant difference.The results suggest that estrogen combined with ovariectomy can induce femur osteoporosis rapidly in rats. The mechanism may be related to down-regulation of Runx2 and up-regulation of mRNA expression of type I collagen 伪 1 and peroxisome proliferator activated receptor 纬.
【作者单位】：广州中医药大学;广州中医药大学第一附属医院;
【基金】：国家自然科学基金(81503591、81674000) 广东省自然科学基金(2014A030310082) 广东省科学技术厅-广东省中医药科学院联合科研项目(2016A030313645,2014A020221021) 广州中医药大学第一临床医学院优秀博士学位论文培育项目(YB201501,YB201602) 广州中医药大学第一附属医院创新强院项目(2015QN03)~~
【分类号】：R-332;R580

【相似文献】