大鼠肺组织缺血再灌注损伤后microRNA的表达变化及意义

发布时间：2018-04-04 07:09

  本文选题：microRNA　切入点：缺血再灌注　出处：《青岛大学》2012年硕士论文

【摘要】：目的：检测大鼠肺组织缺血再灌注损伤过程中后microRNA(miRNA)的表达变化,探讨microRNA与肺组织缺血及再灌注损伤的相关性。 方法：采用改良Eppinger描述的大鼠肺缺血再灌注损伤动物模型,将25只大鼠随机分为五组,分别为对照组、缺血组(1h)与缺血再灌注组(1h、3h、6h三个亚组)。应用基因芯片技术(microarray)检测对照组、缺血组与缺血再灌注组肺组织中microRNA表达水平。运用stem-loop RT-qPCR法检测芯片结果明显变化的4个microRNA在对照组、缺血组(1h)与缺血再灌注组(1h、3h、6h三个亚组)五组中的表达水平。动态观察大鼠肺缺血再灌注损伤急性期microRNA的变化。 结果：1.基因芯片技术(microarray)检测正常组与缺血组肺组织中390个microRNA表达水平变化,与对照组相比,缺血肺组织中有4个miRNA表达上调两倍及两倍以上,21个miRNA表达下调两倍及两倍以上。 2.运用stem-loop RT-qPCR检测法检测(?)miR-1、miR-18a、miR-31和miR-451在对照组与实验组的肺组织中的表达水平,发现在对照组、缺血组及再灌注组的各个时间点miR-18a表达先上调再下调；miR-31表达先下调再上调再下调,在再灌注1h达到最低点,再灌注3h表达水平最高；miR-451表达先上调再下调,在再灌注3h达到最高点。 结论：大鼠肺组织急性缺血期,多个miRNA表达发生变化,提示miRNA参与肺缺血损伤过程。与调亡相关的miR-1、miR-31、miR-451在肺缺血再灌注过程中表达上调或下调,提示miRNA参与肺缺血再灌注损伤的机制与细胞凋亡密切相关。与血管生成抑制相关的miR-18a在肺缺血再灌注过程中表达上调或下调,提示miRNA参与肺缺血再灌注损伤的机制与血管生成也密切相关。
[Abstract]:Objective: to detect the expression of microRNA (miRNA) in lung tissue during ischemia-reperfusion injury in rats, and to explore the correlation between microRNA and lung ischemia-reperfusion injury.
Methods: the lung ischemia modified Eppinger described reperfusion injury of rat animal model, 25 rats were randomly divided into five groups, which were control group, ischemia group (1H) and ischemia reperfusion group (1H, 3h, 6h three groups). The application of gene chip technology (microarray) detection and control the expression level of microRNA group, ischemia group and ischemia reperfusion group in the lung tissue. Using stem-loop RT-qPCR method to detect the microarray results obvious change of 4 microRNA in the control group, ischemia group (1H) and ischemia reperfusion group (1H, 3h, 6h three groups) expression levels in the five groups. To observe the dynamic change lung ischemia reperfusion injury in rat acute microRNA.
Results: 1.. Gene chip technology (microarray) was used to detect 390 microRNA expression levels in lung tissue of normal group and ischemic group. Compared with control group, 4 miRNA expression increased two times and two times higher than that of control group, 21 miRNA expression was down two times and two times.
2. using stem-loop RT-qPCR to detect (?) miR-1, miR-18a, the expression level of miR-31 and miR-451 in the experimental group and the control group in the lung tissues, found in the control group, ischemia group and reperfusion group at each time point miR-18a was upregulated first and then down; the expression of miR-31 under the first tune and then raised down again, to the lowest point at 1h of reperfusion, 3h of reperfusion, the highest expression level; the expression of miR-451 increased firstly and then down, reached the highest point at 3h after reperfusion.
Conclusion: the lung tissue of rats with acute ischemia, multiple miRNA expression changes, suggesting that miRNA involved in lung ischemia. And the apoptosis related miR-1, miR-31, expression of miR-451 in lung ischemia reperfusion process, suggesting that miRNA in lung ischemia reperfusion injury and the mechanism of apoptosis is closely related with. Inhibition of angiogenesis related miR-18a expression in lung ischemia reperfusion process, suggesting that miRNA in lung ischemia reperfusion injury and mechanism of vascular formation are closely related.

【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

【共引文献】

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2 石彦超;施明;刘振文;王福生;;microRNA在肝移植研究中的进展[J];肝脏;2013年10期

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1 刘芬;缺氧诱导miRNA调控肾缺血后血管新生的分子机制[D];南昌大学;2011年

2 张毅;腹部创伤应激后血清microRNA表达改变及其意义[D];第四军医大学;2011年

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2 邓君;肾缺血再灌注损伤后miR-210及其靶基因的变化[D];南昌大学;2010年

3 刘国岩;肝脏缺血再灌注损伤的评估及防护[D];安徽医科大学;2013年

4 黄峰;风湿性心脏病伴房颤患者心房肌microRNA差异表达的观察[D];中南大学;2013年

5 李俊平;MicroRNA-24及其靶基因GATA-2在肾缺血再灌注损伤的表达变化[D];华中科技大学;2013年

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