PT8A的粘膜免疫佐剂活性初步研究

发布时间：2018-04-04 06:28

本文选题：PTA　切入点：LTBELISA　出处：《基因组学与应用生物学》2017年10期

【摘要】：本研究发现位于大肠杆菌不耐热肠毒素B亚单位(heat-labile enterotxin B subunit,LTB)的8肽(octapetptide,PT8A)具有一定的佐剂活性。我们用PT8A联合人手足口病病毒的VP1(EVP1)鼻腔免疫Balb/c小鼠,通过间接ELISA法检测小鼠血清中的特异性EVP1抗体滴度,检测结果显示PT8A+EVP1组相比PBS组和EVP1组有明显佐剂活性,初步验证了PT8A的佐剂活性。对PT8A和LTB蛋白的佐剂活性进行比较研究,结果显示,PT8A的佐剂活性明显弱于LTB,有待进一步提高。通过对PT8A免疫原性进行检测分析发现,PT8A的免疫原性明显减弱,初步确定PT8A在保留佐剂活性的同时自身免疫原性明显减弱,具有进一步开发为粘膜免疫佐剂候选分子的潜力。
[Abstract]:In this study, it was found that octapetptide PT8A, an octapetptide PT8A, which is located in the subunit of heat-labile enterotxin B subunit LTB, has a certain adjuvant activity.Balb/c mice were immunized with PT8A combined with human HFMD virus (VP1EVP1) nasal cavity. The titer of specific EVP1 antibody in serum was detected by indirect ELISA method. The results showed that PT8A EVP1 group had significant adjuvant activity compared with PBS group and EVP1 group.The adjuvant activity of PT8A was preliminarily verified.The adjuvant activity of PT8A and LTB protein was compared. The results showed that the adjuvant activity of PT8A was obviously weaker than that of LTB, which needed to be further improved.Through the detection and analysis of PT8A immunogenicity, it was found that the immunogenicity of PT8A was obviously weakened. It was preliminarily confirmed that PT8A had the potential to be a candidate molecule for mucosal immune adjuvant while preserving its adjuvant activity.
【作者单位】：重庆医科大学;分子医学与肿瘤研究中心;
【基金】：病原微生物生物安全国家重点实验室开放课题(SKLPBS1523)资助
【分类号】：R392

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