建立一种新型的评价大肠杆菌在宿主体内生长及对宿主防御反应差异的小鼠模型

发布时间：2018-04-06 21:19

本文选题：细菌　切入点：宿主　出处：《免疫学杂志》2017年08期

【摘要】：目的建立一种新型的与临床相关的研究动物免疫反应的体系,该体系能够使研究者在同一宿主体内观察到不同基因型细菌对抗机体免疫的差异,为致病微生物的防治提供新的思路。方法首先向小鼠注射环磷酰胺构建粒缺小鼠模型,再向小鼠腹腔注射不同基因型的大肠杆菌模式菌株Escherichia coli K-12的2株混合菌株,一定时间后将小鼠处死并取小鼠各组织液,将这些样品涂布,长出的每个菌落先后经过菌落PCR以及荧光显微镜观察来确定其基因型,并统计2种菌的比例。结果小鼠腹腔注射环磷酰胺后外周血中性粒细胞降至原来水平的10%左右,骨髓中性粒细胞降至原水平的5%左右;向粒缺小鼠腹腔按照1∶1比例注射不同基因型的2株E.coli K-12的菌后,在第3天二者在小鼠腹腔以及各脏器内的比值从原先的1左右升高到了5左右,且有显著性差异(P0.01);而向粒缺小鼠腹腔按照1∶1比例注射2株不同荧光蛋白标记的相同的E.coli K-12野生型菌株后,2株菌在小鼠体内的存活并没有明显的差异(保持在1左右)。结论我们建立的这种新型免疫反应评价体系能够准确反应出不同基因型细菌在粒缺的小鼠体内对抗机体免疫的能力。此体系中,实验菌株和对照菌株同时注射到同一受鼠,很好的解决了小鼠个体间差异的问题,这种新的实验系统的建立对研究放化疗患者的免疫应答机制和抗感染治疗策略的制定具有重要指导意义。
[Abstract]:Objective to establish a new clinical system to study the immune response of animals, which enables researchers to observe the differences between different genotypes of bacteria against immune responses in the same host.To provide a new idea for the prevention and treatment of pathogenic microorganisms.Methods the mice were injected with cyclophosphamide to construct a mouse model of hypogranulocyte deficiency, and then two mixed strains of E. coli model strain Escherichia coli K-12 with different genotypes were injected intraperitoneally. The mice were killed and the tissues of the mice were collected after a certain period of time.The samples were coated and each colony was observed by colony PCR and fluorescence microscope to determine the genotypes and to calculate the proportion of the two strains.Results after intraperitoneal injection of cyclophosphamide, neutrophils in peripheral blood and bone marrow neutrophils decreased to about 10% and 5%, respectively.Two strains of E.coli K-12 with different genotypes were injected into the abdominal cavity of mice with different genotypes at 1:1. On the third day, the ratio of E.coli K-12 in the abdominal cavity and organs of the mice increased from about 1 to about 5.Conclusion the new immune response evaluation system developed by us can accurately reflect the ability of different genotypes of bacteria to resist immunity in mice with hypogranulocytes.The establishment of this new experimental system is of great significance for the study of the immune response mechanism and the formulation of anti-infection treatment strategy in patients with radiotherapy and chemotherapy.
【作者单位】：中国医学科学院血液学研究所实验血液学国家重点实验室血液学研究所;中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室;
【基金】：国家自然科学基金(31471116,81600083) 中国医学科学院医学科学创新基金(2016-PM-1-003)
【分类号】：R-332;R392

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