真武汤对心力衰竭模型大鼠心室重构及心肌细胞凋亡、纤维化的影响

发布时间：2018-04-08 07:32

本文选题：心力衰竭　切入点：真武汤　出处：《中医杂志》2017年14期

【摘要】：目的探讨真武汤治疗心力衰竭的可能作用机制。方法将50只Wistar大鼠随机分为假手术组、模型组及真武汤低、中、高剂量组,每组10只。除假手术组外其余各组采用结扎腹主动脉方法建立心力衰竭模型。真武汤低、中、高剂量组分别给予真武汤6、12、18 g/(kg·d)灌胃,假手术组、模型组分别给予蒸馏水3 ml灌胃,每天1次,连续给药28天。给药后各组大鼠行高频超声心动图检查,测定左心室舒张末期内径(LVIDD)、左心室收缩末期内径(LVESD)、左心室射血分数(LVEF)及左心室短轴缩短指数(FS)。TUNEL法检测并计算各组大鼠心肌细胞凋亡指数;ELISA法检测各组大鼠心肌组织Ⅰ型胶原蛋白(ColⅠ)、Ⅲ型胶原蛋白(ColⅢ)表达;Real-time PCR检测各组大鼠心肌组织骨膜蛋白、转化生长因子β1(TGF-β1)、葡萄糖调节蛋白78(GRP78)、C/EBP环磷酸腺苷反应元件结合转录因子同源蛋白(CHOP)mRNA表达;Western blot法检测各组大鼠心肌组织骨膜蛋白、TGF-β1、GRP78、CHOP蛋白表达。结果与模型组比较,真武汤高、中、低剂量组心脏超声各项指标、心肌纤维化程度均明显改善,心肌凋亡细胞显著减少,ColⅠ、ColⅢ表达,骨膜蛋白、TGF-β1、GRP78、CHOP蛋白及mRNA表达均明显降低,且真武汤高剂量组改善最为明显(P0.05)。结论真武汤可改善心力衰竭大鼠心室重构,减少心肌细胞凋亡和心肌纤维化,可能是其治疗心力衰竭的机制之一。
[Abstract]:Objective to explore the possible mechanism of Zhenwu Decoction in the treatment of heart failure. Methods: 50 Wistar rats were randomly divided into sham operation group, model group and Zhenwu Decoction low, high dose group, 10 rats in each group. Except the sham operation group, other groups were made by abdominal aortic ligation method to establish the model of heart failure. In Zhenwu Decoction low., high dose groups were given Zhenwu Decoction 6,12,18 (kg - D) g/ gavage, sham operation group, model group were given distilled water 3 ml orally, 1 times a day, continuous administration for 28 days. After administration, the rats underwent high frequency echocardiography for measuring left ventricular end diastolic diameter (LVIDD), left ventricular end systolic diameter (LVESD), left ventricular ejection fraction (LVEF) and left ventricular shortening index (FS) detected by.TUNEL and calculated the index of rats myocardial cell apoptosis; detection of myocardial tissue of rats of type I collagen ELISA (Col 1), type III collagen (Col III) The expression of Periostin; detection of myocardial tissue of rats Real-time PCR, transforming growth factor beta 1 (TGF- beta 1), glucose regulated protein 78 (GRP78), C/EBP cAMP response element binding transcription factor homologous protein (CHOP) expression of mRNA; detection of Periostin myocardial tissue of rats by blot Western, TGF- beta 1 GRP78, CHOP, protein expression. Results compared with the model group, Zhenwu Decoction, high index of echocardiography in low dose group, the degree of myocardial fibrosis were significantly improved, myocardial apoptosis cells were decreased significantly, Col I, Col III expression of bone, membrane protein, TGF- beta 1, GRP78, the expression of CHOP protein and mRNA were significantly reduced, and Zhenwu Decoction high dose group was improved significantly (P0.05). Conclusion Zhenwu Decoction can improve ventricular remodeling in rats with heart failure, myocardial fibrosis and myocardial cell apoptosis, which may be the treatment of heart failure mechanism.

【作者单位】：江西中医药大学附属医院;
【基金】：国家自然科学基金(8140680)
【分类号】：R285.5;R-332

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