颈动脉注射血吸虫虫卵建立兔脑肉芽肿模型的研究

发布时间：2018-04-09 17:41

本文选题：脑肉芽肿　切入点：动物模型　出处：《中国病原生物学杂志》2017年09期

【摘要】：目的通过颈动脉注射血吸虫虫卵建立家兔脑肉芽肿模型。方法实验组家兔10只,行全脑放疗(20GY,单次照光)破坏血脑屏障;照光后7d,通过颈动脉注射血吸虫虫卵悬浮液1ml建模。阴性对照组10只,全脑放疗后7d经颈动脉注射生理盐水1ml。空白对照组10只,不进行任何处理。术后使用抗生素预防感染,观察3组动物临床表现。30d后行MRI检查。取脑组织做病理切片,观察脑组织大体标本及病理改变。结果实验组2只、阴性对照组1只家兔死于放疗后并发症。实验组8只家兔术后均出现轻度食欲不振,体重下降,1周后症状逐渐消失;8只出现呆滞、嗜睡等精神症状。阴性对照组家兔术后均食欲下降,1周后症状消失,无异常精神症状。空白对照组未见异常。MRI检查实验组增强T1WI见脑膜线状强化4例,脑组织内见结节状强化2例。对照组未见明显异常。脑组织大体标本及病理切片检查实验组8只家兔均出现血管炎,7只出现脑水肿,6只病理切片见肉芽肿结节,5只出现脑膜炎。阴性对照组及空白对照组未见脑水肿及肉芽肿结节(阴性对照组1只出现血管周围炎)。结论经颈动脉注射血吸虫虫卵建立的家兔脑肉芽肿模型出现相应的组织病理变化及临床表现,符合实验要求。
[Abstract]:Objective through carotid artery injection of schistosome eggs to establish cerebral granuloma model in rabbits. Methods the experimental group of 10 rabbits underwent whole brain radiotherapy (20GY, single light) disruption of the blood-brain barrier; illumination of 7D, through carotid artery injection of Schistosoma japonicum egg suspension 1ml modeling. The negative control group 10, whole brain radiotherapy 7d carotid artery injection of saline 1ml. 10 rats in the blank group, without any treatment. Postoperative use of antibiotics to prevent infection, observe 3 groups of animal clinical manifestations of.30d MRI was performed. The brain tissue was taken for pathological observation of brain tissue specimens and pathological changes. Results in the experimental group, negative control group 2, 1 rabbits died of complication after radiotherapy. The experimental group of 8 rabbits after operation showed mild loss of appetite, weight loss, 1 weeks after the symptoms gradually disappeared; 8 will appear dull, mental symptoms of sleepiness. Negative control group rabbits after operation were decreased appetite, 1 weeks after the symptoms disappear Lost, no abnormal mental symptoms. The blank control group showed no abnormal.MRI examination of the experimental group enhanced T1WI linear meningeal enhancement in 4 cases, brain tissue in nodular enhancement in 2 cases. The control group had no obvious abnormalities. Brain tissue specimens and pathological examination of the experimental group of 8 rabbits showed vasculitis, 7 appeared cerebral edema. 6 pathological sections showed granulomatous nodules, 5 meningitis. The negative control group and blank control group had no brain edema and granuloma nodules (negative control group 1 rats showed perivascular inflammation). Rabbit cerebral granuloma model conclusion through carotid artery injection of schistosome eggs established corresponding histopathologic changes and clinical manifestations, in line with the experimental requirements.

【作者单位】：江南大学附属医院;江南大学公共卫生学院;江苏省血吸虫病防治研究所;浙江中医药大学动物实验研究中心;浙江大学附属第二医院放疗科;江南大学医学院;
【基金】：江南大学公共卫生研究中心立项项目(No.1286010242150640)
【分类号】：R-332;R532.21

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