Gαi2在大鼠脑缺血再灌注损伤模型海马中的表达及其对神经细胞内钙离子浓度的影响

发布时间：2018-04-11 01:01

本文选题：Gαi2 + 脑缺血再灌注损伤　；参考：《华中科技大学》2012年硕士论文

【摘要】：目的探讨分析抑制性G蛋白α亚单位Gαi2在大鼠脑缺血再灌注损伤中的表达变化和对神经细胞内钙离子浓度和凋亡的影响。方法将90只SD大鼠随机分为假手术组(SS组，n=30)、脑缺血再灌注组(IR组，n=30)、脑缺血再灌注且脑室内百日咳毒素（PT）处理组(PT组，n=30)，免疫组化、westernblotting分别测定缺血再灌注后6h、12h、24h各组中海马神经内Gαi2的存在及表达，流式细胞术测定各组中海马细胞内游离Ca~（2+）浓度的平均荧光值，分析比较它们之间的关系，TUNEL法检测神经元细胞凋亡。结果相对应时间点，IR组Gαi2含量和Ca~（2+）浓度较SS组明显增高(P＜0.01)，PT组Gαi2表达量较IR组无显著变化(P0.05)，，PT组Ca~（2+）浓度较IR组升高(P＜0.01)，PT组神经细胞凋亡率较IR组升高(P＜0.05)。论Gαi2在大鼠脑缺血再灌注损伤中表达明显增高，并可降低缺血神经细胞内的钙离子浓度，减少神经细胞的凋亡，对缺血神经细胞具有保护作用。
[Abstract]:Objective to investigate the expression of inhibitory G protein 伪 subunit G 伪 I 2 in cerebral ischemia-reperfusion injury in rats and its effect on intracellular calcium concentration and apoptosis.Methods 90 Sprague-Dawley rats were randomly divided into three groups: sham operation group (SS group), cerebral ischemia reperfusion group (IR group), cerebral ischemia reperfusion group (IR group), cerebral ischemia reperfusion group (PT group) and intraventricular pertussis toxin (PTT) treatment group (n = 30).The presence and expression of G 伪 I 2 in hippocampal nerve,Flow cytometry (FCM) was used to measure the mean fluorescence of free Ca~(2 in hippocampal cells. The relationship between them was analyzed and Tunel was used to detect the apoptosis of neurons.The expression of G 伪 I 2 in cerebral ischemia-reperfusion injury in rats was significantly increased, and the Ca 2 + concentration in ischemic nerve cells was decreased, the apoptosis of nerve cells was reduced, and the protective effect of G 伪 I 2 on ischemic nerve cells was observed.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332;R651.12

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