猪、鸡源分离粪肠球菌的核糖体分型及耐药性分析

发布时间：2018-04-12 21:52

本文选题：粪肠球菌 + 耐药性　；参考：《中国抗生素杂志》2017年03期

【摘要】：对上海地区1个规模化养猪场和1个规模化养鸡场分离鉴定的4 0株粪肠球菌,采用微量肉汤稀释法对12种抗菌药物进行药敏试验,采用Riboprinter~?微生物基因指纹鉴定系统检测分离菌株的基因指纹图谱,分别检测分离菌株中的核糖体型和耐药性。结果表明,在40株粪肠球菌中,80%以上的为多重耐药菌株,有4株猪源粪肠球菌对万古霉素耐药,其中有2株表现为对万古霉素高度耐药(MIC64mg/L),且对链霉素和庆大霉素同时表现为高度耐药(MIC2048mg/L)。从耐药表型和核糖体分型共同分析粪肠球菌的耐药性,发现多重耐药性严重,同一核糖体型菌株的耐药表型并非完全一致。
[Abstract]:Forty strains of Enterococcus faecalis isolated and identified from a large scale pig farm and a large scale chicken farm in Shanghai area were tested for drug sensitivity of 12 antimicrobial agents by the method of broth dilution and Riboprinterum?Microorganism gene fingerprint system was used to detect the gene fingerprint of the isolated strain, and the ribosomal type and drug resistance of the isolated strain were detected respectively.The results showed that more than 80% of the 40 strains of Enterococcus faecalis were multidrug resistant, and 4 strains of Enterococcus faecalis were resistant to vancomycin.Two of them showed high resistance to vancomycin, MIC64mg / L, and to streptomycin and gentamicin, they were highly resistant to MIC2048mg / L, respectively.The drug resistance of Enterococcus faecalis was analyzed from the drug resistance phenotype and ribosomal typing. It was found that the multidrug resistance of Enterococcus faecalis was serious and the resistance phenotype of the same ribosomal strain was not identical.
【作者单位】：上海市兽药饲料检测所;
【基金】：上海市科技兴农重点攻关项目(沪农科攻字(2015)第6-3-1号)
【分类号】：S852.61

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