鼠疫F1抗体检测金标试纸条样本稀释液的筛选

发布时间：2018-04-12 20:57

本文选题：鼠疫 + F抗体　；参考：《现代预防医学》2017年20期

【摘要】：目的为鼠疫F1抗体检测金标试纸条筛选出较为合适的样本稀释液,以有效避免其非特异性反应,提高试纸条的检测质量。方法选取4种血清样本稀释液,对同一份鼠疫菌免疫兔血清稀释后作鼠疫F1抗体检测,筛选出不影响敏感性的稀释液;用该稀释液检测既往鼠疫患者血清、既往发生非特异反应的血清及现场样本,验证该稀释液的检测效果。结果 4种稀释液检测同一份鼠疫菌免疫兔血清的最高检出滴度无明显差异;用含0.02 mol/L磷酸盐缓冲液(PBS)pH7.4、含0.5%牛血清白蛋白第五组份(ABV)生理盐水、含5%兔血清生理盐水对比检测10份既往鼠疫患者血清检测均为阳性;用含0.5%ABV生理盐水检测37份既往发生非特异反应血清和293份现场采集犬血清均为阴性。结论含0.5%ABV生理盐水能有效避免胶体金免疫层析法(GICA)非特异性反应的发生而不影响敏感性,可作为鼠疫F1抗体检测金标试纸条的稀释液。
[Abstract]:Objective to screen out a more suitable sample diluent for the detection of plague F1 antibody with gold standard test strip in order to effectively avoid its nonspecific reaction and improve the quality of the test strip.Methods four kinds of diluents of serum samples were used to detect the F1 antibody of Yersinia pestis after dilution of the same rabbit serum immunized with Yersinia pestis, and the diluent was used to detect the sera of the previous plague patients, which did not affect the sensitivity of the sera of the rabbits immunized with Yersinia pestis.The serum and field samples of non-specific reaction in the past were used to verify the detection effect of the diluent.Results there was no significant difference in the titer of the four diluents in detecting the highest titer of the same rabbit serum immunized with Yersinia pestis. The pH value of PBS7. 4 and the fifth component of bovine serum albumin (BSA) were 0.5% in the phosphate buffer solution containing 0.02 mol/L.10 cases of Yersinia pestis were tested positive by comparison of normal saline with 5% rabbit serum, 37 cases of nonspecific reaction serum with 0.5 V saline and 293 samples of dog serum were negative.Conclusion 0.5 V normal saline can effectively avoid the occurrence of non-specific reaction of colloidal gold immunochromatography (GICA) and does not affect the sensitivity. It can be used as a diluent for the detection of gold standard test strip by plague F1 antibody.
【作者单位】：云南省地方病防治所云南省鼠疫防治研究中心云南省自然疫源性疾病防控技术重点实验室;
【基金】：国家自然科学基金项目(No.81360413) 云南省技术创新人才培养项目(2014HB093)
【分类号】：R392-33

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