一株含有5个原噬菌体的单增李斯特菌比较基因组分析

发布时间：2018-04-13 09:48

本文选题：单增李斯特菌 + 比较基因组学　；参考：《基因组学与应用生物学》2017年02期

【摘要】：单增李斯特菌是一种重要的人畜共患病原菌,对食品安全和人类健康造成了严重威胁,但现阶段对其进化规律的研究仍相对较少。本研究运用Illumina测序技术对一株中国单增李斯特菌WaX12的基因组进行了测定,并通过比较基因组学分析了该菌的微进化规律。结果表明:WaX12的基因组与Finland 1998相似度最高,但两者基因组间存在135个基因的差异,主要集中在WaX12的原噬菌体上。进一步分析可知,WaX12的基因组携带5个原噬菌体,除原噬菌体1外,其余4个原噬菌体均未存在于Finland 1998中。原噬菌体3和原噬菌体5来源于其他单增李斯特菌的基因组,而原噬菌体2和原噬菌体4则分别来自于大肠杆菌和粪球菌。由此可见,原噬菌体是该单增李斯特菌微进化的主要原因,而这种微进化的发生不仅限于同一种属的细菌之间,还可能源于不同种属细菌间的基因转移。
[Abstract]:Listeria monocytogenes (Listeria monocytogenes) is an important zoonotic pathogen which poses a serious threat to food safety and human health.In this study, the genome of a Chinese Listeria monocytogenes WaX12 was determined by Illumina sequencing, and its microevolution was analyzed by comparative genomics.The results showed that the genome of 1: WaX12 had the highest similarity to that of Finland 1998, but the difference of 135 genes between the two genomes was mainly concentrated on the prophage of WaX12.Further analysis showed that the genome of WaX12 carried 5 prophages, except for prophage 1, none of the other 4 prophages existed in Finland 1998.Prophage 3 and prophage 5 were derived from the genomes of other Listeria monocytogenes, while prophage 2 and phage 4 were derived from Escherichia coli and faecal coccus, respectively.Therefore, the bacteriophage is the main reason for the microevolution of Listeria monocytogenes, and this microevolution is not only limited to the same species of bacteria, but also may be due to gene transfer between different species of bacteria.
【作者单位】：上海海洋大学食品学院;农业部水产品贮藏保鲜质量安全风险评估实验室;浙江工业大学生物与环境工程学院;上海交通大学微生物代谢重点实验室与生命科学和生物技术学院;
【基金】：国家自然基金(31271870) 上海市科委项目(14DZ1205100,14320502100) 上海农业科技发展重点项目(2014,3-5;2015,4-8) 上海海洋大学优秀研究生培育计划共同资助
【分类号】：R378;Q78

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