E-cad在低位直肠癌淋巴结转移裸鼠模型血清及移植瘤中的表达及其意义

发布时间：2018-04-15 22:38

本文选题：直肠癌 + 淋巴结转移　；参考：《中国比较医学杂志》2017年03期

【摘要】：目的探讨裸鼠低位直肠癌淋巴结转移模型血清及移植瘤组织中E-钙黏蛋白(E-cad)的表达情况,分析其在直肠癌淋巴结转移过程中的作用。方法取对数生长期的人大肠癌细胞株SW480接种于裸鼠直肠黏膜下,建立裸鼠低位直肠癌淋巴结转移模型。120只裸鼠随机分为3组,每组40只,A组(直肠癌模型组)采用二甲基肼注射+SW480种植,B组(二甲基肼对照组)采用二甲基肼注射,C组(空白对照组)不作任何处理。采用ELISA检测血清E-cad的含量,采用Western blot法、RT-PCR法检测种植瘤组织中E-cad蛋白及mRNA表达水平。结果A组裸鼠的血清E-Cad含量为(19.48±1.25)mg/L,明显高于B组裸鼠和C组裸鼠的血清E-Cad含量(2.36±0.18mg/L和2.15±0.12 mg/L)(t=8.28,9.01,P0.05)。发现侧方淋巴结转移的裸鼠血清E-Cad含量,明显高于未发现侧方淋巴结转移的裸鼠,差异有显著性(t=10.28,P0.05)。A组裸鼠移植瘤组织中E-Cad蛋白的表达强度弱于B组和C组(t=9.81,7.69,P0.05)。A组中,有侧方淋巴结转移发生的裸鼠移植瘤组织中E-Cad蛋白表达强度较未有侧方淋巴结转移发生的裸鼠移植瘤组织中表达明显减弱,差异有显著性(t=9.36,P0.05)。A组裸鼠移植瘤组织中E-Cad mRNA表达明显下调,与B组和C组比较,有明显的差异(P0.05)。A组中发现侧方淋巴结转移的裸鼠移植瘤组织中E-Cad mRNA表达强度明显下调,与未发现侧方淋巴结转移的裸鼠比较,差异有显著性(t=7.85,P0.05)。结论血清E-cad的表达水平可能与直肠癌淋巴转移密切相关,同时直肠癌组织中E-Cad蛋白和mRNA表达减弱或缺失,导致癌细胞间粘附性降低,促进癌细胞获得侵袭和转移的能力。
[Abstract]:Objective to investigate the nude mice of low rectal cancer lymph node metastasis model of E- calcium sticky protein in serum and tumor tissue (E-cad) expression and analysis of its function in the process of metastasis of rectal cancer lymph. The logarithmic growth of the human colon cancer cell line SW480 in nude mice of rectal mucosa, rectal cancer lymph node metastasis in nude mice model.120 mice were randomly divided into 3 groups, 40 rats in each group, A group (cancer model group) with two methyl hydrazine injection of +SW480 plant, B group (two methyl hydrazine group) with two methyl hydrazine injection, C group (control group) without any treatment. The content of serum E-cad ELISA, the Western blot method, RT-PCR method was used to detect the level of E-cad plant protein and mRNA expression in tumor tissue. Results the serum E-Cad content of group A in nude mice for (19.48 + 1.25) mg/L, significantly higher than that of B group and C group of nude mice in nude mice serum E-Cad content (2.36 + 0.18mg 2.15 + 0.12) /L and mg/L (t=8.28,9.01, P0.05). It is found that the lateral lymph node metastasis in nude mice serum E-Cad content was significantly higher than that found no lateral lymph node metastasis in nude mice, there was a significant difference (t=10.28, P0.05) expression of E-Cad protein in tumor tissues of nude mouse in.A group was less than that of B group and C group (t=9.81,7.69, P0.05) in the.A group, significantly decreased expression intensity is no lateral lymph node metastasis occurred in the tumor tissues E-Cad protein expression of metastasis in xenograft lateral lymph nodes, there were significant differences (t=9.36, P0.05) significantly decreased E-Cad mRNA group the expression of.A in tumor tissues of nude mouse, comparison with the B group and C group had significant difference (P0.05) intensity of E-Cad was significantly down regulated mRNA expression of lateral lymph node metastasis in nude mice transplanted tumor tissue found in the.A group, compared with lateral lymph node metastasis in nude mice and found that there were significant differences (t=7.85 P0.05). Conclusion the expression level of serum E-cad may be closely related to lymph node metastasis of rectal cancer. Meanwhile, the expression of E-Cad and mRNA in rectal cancer tissue is weakened or absent, which leads to the decrease of intercellular adhesion and the promotion of invasion and metastasis of cancer cells.

【作者单位】：新疆医科大学第一附属医院肿瘤中心;新疆医科大学第五附属医院普外科;
【基金】：新疆维吾尔自治区自然科学基金(项目编号:2014211C131)
【分类号】：R735.37;R-332

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