聚乙二醇化Exendin-4类似物受体亲和力测定方法的建立

发布时间：2018-04-16 03:21

本文选题：Exendin- + 聚乙二醇化　；参考：《中国药科大学学报》2016年02期

【摘要】：基于荧光素酶报告基因法和本实验室前期构建的CHO-GLP-1R-CRE-Luc+检测模型细胞株,建立并优化了长效降糖多肽——聚乙二醇化Exendin-4类似物PE与GLP-1受体的亲和力测定方法,并对其进行了方法学验证。结果表明,当药物作用4 h,化学发光底物作用15 min,检测药物在5.7×10-3~1.5×103nmol/L浓度区间内时,该检测方法的专属性和耐用性良好、准确度和精密度较高,符合《生物制品质量控制分析方法验证技术一般原则》的相关要求。本研究为该类GLP-1受体激动剂药物的快速评价与筛选奠定了基础。
[Abstract]:Based on the luciferase reporter gene method and the CHO-GLP-1R-CRE-Luc detection model cell line constructed in our laboratory, the affinity of the long-acting hypoglycemic polypeptide-polyethylene glycol Exendin-4 analogue PE to GLP-1 receptor was established and optimized.The methodology was validated.The results showed that the specificity and durability of the method were good, the accuracy and precision were high when the chemiluminescence substrate was exposed to the chemiluminescent substrate for 4 h and the detection drug was in the concentration range of 5.7 脳 10 ~ (-3) ~ (-1) 脳 1. 5 脳 103nmol/L.Accord with the requirements of the General principle of validation Technology for quality Control Analysis of Biological products.This study laid a foundation for rapid evaluation and screening of GLP-1 receptor agonists.
【作者单位】：中国药科大学生命科学与技术学院;
【基金】：国家“重大新药创制”科技重大专项资助项目(No.2012ZX09103101-016) 教育部博士点基金资助项目(No.20120096110007)~~
【分类号】：R392-33

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