下调CDH17基因对那可丁耐药性人胃癌细胞裸鼠移植瘤模型的影响

发布时间：2018-04-16 13:15

  本文选题：胃癌 + CDH基因　； 参考：《现代免疫学》2017年02期

【摘要】：研究下调CDH17基因对那可丁耐药的人胃癌MGC-803细胞裸鼠移植瘤模型的影响,为临床上寻求治疗胃癌新方案提供理论依据。以人胃癌MGC-803细胞作为研究材料,建立该细胞的裸鼠移植瘤模型。同时,用HE染色法观察瘤体组织病理学形态。结果显示,RT-PCR和Western blotting实验中siCDH17慢病毒对胃癌MGC-803细胞中CDH17的转录水平和蛋白质表达水平均具有干扰效果(P0.05)。随后将siCDH17慢病毒干扰的人胃癌MGC-803细胞以及对照细胞进行裸鼠皮下移植瘤实验。移植后第6天各组均见皮下肿瘤结块,采用那可丁药物处理并实时检测肿瘤生长情况。结果发现,siCDH17组肿瘤体积和质量明显小于对照组和siNC组(P0.05)。且HE染色也发现与对照组比较,siCDH17组的瘤体肿瘤细胞生长受到抑制。因此,下调CDH17基因可以增加胃癌MGC-803细胞对那可丁的敏感性,抑制胃癌MGC-803细胞的生长,最终阻滞裸鼠移植瘤的生长。
[Abstract]:To study the effect of down-regulation of CDH17 gene on the xenograft tumor model of human gastric cancer MGC-803 cells in nude mice with drug resistance of nakodin, and to provide a theoretical basis for seeking a new regimen for the treatment of gastric cancer in clinic.The xenograft tumor model of human gastric cancer MGC-803 cells was established in nude mice.At the same time, the histopathological morphology of the tumor was observed by HE staining.The results showed that siCDH17 lentivirus in RT-PCR and Western blotting could interfere with the transcription and protein expression of CDH17 in MGC-803 cells of gastric cancer.Then human gastric cancer MGC-803 cells and control cells interfered with siCDH17 lentivirus were subcutaneously transplanted into nude mice.On the 6th day after transplantation, subcutaneous tumor caking was observed in all groups.The results showed that the tumor volume and mass in siCDH17 group were significantly lower than those in control group and siNC group (P 0.05).He staining also showed that tumor cell growth of siCDH 17 group was inhibited compared with control group.Therefore, down-regulation of CDH17 gene can increase the sensitivity of gastric cancer MGC-803 cells to nakodin, inhibit the growth of gastric cancer MGC-803 cells, and eventually block the growth of xenografts in nude mice.
【作者单位】： 武汉市第三医院消化内科;
【基金】：湖北省自然科学基金(2013CFB358) 武汉市卫计委(WX14C18)
【分类号】：R735.2;R-332
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本文编号：1759093