CXCR4-NMDAR1偶联—生理电场诱导大鼠神经干细胞定向迁移新机制

发布时间：2018-04-17 22:36

本文选题：电场 + CXCR4　；参考：《重庆医科大学》2012年硕士论文

【摘要】：目的探讨生理强度的电场(250mv/mm)刺激对大鼠胎鼠神经干细胞受体CXCR4和NMDAR1的表达及相互作用的影响,并研究其可能的作用机制。方法体外分离培养大鼠胎鼠的神经干细胞并施加电场,免疫荧光化学方法鉴定神经干细胞；western印迹和细胞免疫荧光化学方法检测电场刺激前后神经干细胞表达CXCR4和NMDAR1的情况；免疫共沉淀和细胞免疫荧光化学方法检测电场刺激前后神经干细胞受体CXCR4和NMDAR1的蛋白偶联情况。western印迹检测其可能的下游信号通路。结果电场刺激可促使大鼠胎鼠神经干细胞表达受体CXCR4和NMDAR1逐渐增多,与电场刺激时间成正比；电场刺激还可促使两受体发生蛋白偶联作用。此外,电场刺激还可使PTEN和Akt的活性发生相应改变。结论生理强度的电场刺激不仅可促使大鼠胎鼠神经干细胞表达神经受体NMDAR1和趋化受体CXCR4上调,还可诱导这两种受体发生蛋白相互偶联作用,并可能通过PTEN/PI3K/Akt信号通路的作用,介导神经干细胞的定向迁移。本研究结果提示,生理强度的电场作用可通过对神经递质受体和化学趋化受体的双重作用,驱动神经干细胞的定向迁移,为临床神经损伤修复的康复治疗提供了一种新的治疗策略。
[Abstract]:Objective to investigate the effects of physiological intensity electric field (250 MV / mm) stimulation on the expression and interaction of neural stem cell receptor (CXCR4) and neural stem cell receptor (NMDAR1) in rat fetal rat neural stem cells (NSCs) and its possible mechanism.Methods Neural stem cells (NSCs) from rat fetal rats were isolated and cultured in vitro and applied electric field. The expression of CXCR4 and NMDAR1 in neural stem cells (NSCs) before and after electric field stimulation was detected by Western blotting and cellular immunofluorescence chemistry.The protein coupling of neural stem cell receptor CXCR4 and NMDAR1 before and after electric field stimulation was detected by immunoprecipitation and immunofluorescence. Western blot was used to detect its possible downstream signal pathway.Results the expression of CXCR4 and NMDAR1 in rat fetal neural stem cells was increased by electric field stimulation, which was in direct proportion to the time of electric field stimulation, and the protein coupling of the two receptors was also induced by electric field stimulation.In addition, the activity of PTEN and Akt can be changed by electric field stimulation.Conclusion the electric field stimulation of physiological intensity can not only up-regulate the expression of neural receptor NMDAR1 and chemotactic receptor CXCR4 in rat fetal neural stem cells, but also induce the interaction between the two receptors, which may be mediated by the PTEN/PI3K/Akt signaling pathway.To mediate the directional migration of neural stem cells.The results suggest that the electric field action of physiological intensity can drive the directional migration of neural stem cells through the dual action of neurotransmitter receptor and chemotactic receptor.It provides a new treatment strategy for the rehabilitation of clinical nerve injury.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

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