巴马小型猪冠状动脉粥样硬化模型的评价方法

发布时间：2018-04-18 04:24

本文选题：模型评价 + 动脉粥样硬化　；参考：《山东大学学报(医学版)》2017年07期

【摘要】：目的从可行性、稳定性和安全性等方面对巴马小型猪冠状动脉粥样硬化模型进行评价。方法巴马小型猪随机分为对照组(给予基础饲料)和模型组(给予高脂喂饲联合机械损伤),在0、24、48周分别对两组行冠状动脉造影、血管内超声(IVUS)、心电图、血脂、炎症指标检测和病理学分析。结果 24周模型组总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、氧化低密度脂蛋白(ox-LDL)、高敏C反应蛋白(hs-CRP)、白介素-6(IL-6)、血管细胞黏附分子-1(VCAM-1)、单核细胞趋化因子-1(MCP-1)和P选择素(s P-selectin)明显高于对照组(P0.05);模型组0、24周ST段下移程度与对照组相比差异无统计学意义(P0.05),模型组0周T波振幅与对照组相比差异无统计学意义(P0.05),模型组24周T波振幅与对照组相比差异有统计学意义(P0.05);24、48周冠状动脉造影和IVUS显示模型组冠脉平均面积狭窄率高于对照组;48周模型组血管内膜厚度和内膜中膜厚度比大于24周。结论离体在体结合评价是一种可行、安全的评价冠状动脉粥样硬化模型的方法。
[Abstract]:Objective to evaluate the feasibility, stability and safety of Bama miniature pig coronary atherosclerosis model.Methods Bama miniature pigs were randomly divided into two groups: control group (basic diet) and model group (hyperlipidemic feeding combined with mechanical injury). Coronary arteriography, intravascular ultrasound IVUSU, electrocardiogram, blood lipid were performed in the two groups at the 48th week of 0 ~ 24h.Detection of inflammatory markers and pathological analysis.Results in the 24-week model group, total cholesterol TCU, triglyceride triglyceride TGG, LDL-Con, HDL-CU, ox-LDLN, Gao Min C-reactive protein hs-CRPN, interleukin-6, interleukin-6, vascular cell adhesion molecule-1 VCAM-1, monocyte chemokine, monocyte chemokine were found in the model group.MCP-1) and P-selectin) were significantly higher than those in control group (P0.05A), but there was no significant difference in St segment depression between model group and control group (P 0.05), but there was no significant difference in T wave amplitude between model group and control group at 0 week (P 0.05) and 24 weeks in model group (P 0.05).The mean area stenosis rate of coronary artery in the model group was higher than that in the control group at 48 weeks after coronary angiography and IVUS. The intima thickness and intima media thickness ratio of the model group were higher than that of the control group at 48 weeks and the ratio of intimal media thickness was more than 24 weeks.Conclusion in vitro combination evaluation is a feasible and safe method to evaluate coronary atherosclerosis model.
【作者单位】：辽宁中医药大学附属医院血脉病研究室;辽宁中医药大学附属医院心内科;辽宁中医药大学实验动物中心;辽宁中医药大学附属医院麻醉科;辽宁中医药大学重大科研平台;
【基金】：国家重点基础研究发展计划(2013CB531704)
【分类号】：R-332;R541.4

【相似文献】