T1DM猕猴肝中CB1R和GR及FAAH的表达

发布时间：2018-04-18 04:38

本文选题：型糖尿病 + 大麻素型受体　；参考：《中国兽医科学》2017年04期

【摘要】：通过研究1型糖尿病(T1DM)猕猴肝内大麻素1型受体(CB1R)、脂肪酸酰胺水解酶(FAAH)和糖皮质激素受体(GR)蛋白和mRNA表达量的变化,探讨内源性大麻素系统对糖尿病猕猴肝的影响。苏木精-伊红染色观察糖尿病猕猴肝的病理变化,均可见肝细胞肿胀伴气球样变,不同程度脂肪变性。油红O染色结果显示,糖尿病猕猴组猕猴肝中脂滴含量显著高于对照组(P0.01)。免疫组织化学结果显示,糖尿病组猕猴肝中CB1R表达量高于对照组(P0.05),而糖尿病组猕猴肝中FAAH、GR表达量均低于对照组(P0.05)。实时荧光定量PCR结果显示,CB1R、GR、FAAH的mRNA的表达量变化与蛋白表达一致,对照组与糖尿病组差异相比有高度统计学意义(P0.01)。结果表明,CB1R、GR、FAAH与T1DM猕猴肝病理损伤密切相关,可为临床上该疾病的监测与治疗提供依据。
[Abstract]:Through the study of type 1 diabetes mellitus (T1DM) in rhesus monkey liver cannabinoid receptor type 1 (CB1R), fatty acid amide hydrolase (FAAH) and glucocorticoid receptor (GR) expression of protein and mRNA, to investigate the effects of endocannabinoid system on diabetic macaque liver. The pathological changes were observed by hematoxylin eosin staining in diabetic macaque liver the liver cells were visible swelling and ballooning, different degrees of fatty degeneration. Oil red O staining showed that the diabetic group in rhesus macaque liver lipid droplet content was significantly higher than the control group (P0.01). The results of immunohistochemistry showed the expression of CB1R was higher than that of the control group, diabetic group of rhesus monkey liver (P0.05), and diabetes mellitus group of monkey liver FAAH GR expression were lower than the control group (P0.05). Real time fluorescence quantitative PCR results showed that CB1R, GR, consistent with the expression of protein expression of FAAH mRNA, compared with the control group and diabetes group height statistics Study significance (P0.01). The results showed that CB1R, GR and FAAH were closely related to the pathological damage of the liver of T1DM rhesus monkeys, which could provide a basis for the clinical monitoring and treatment of the disease.

【作者单位】：四川农业大学动物医学院动物疾病模型实验室;四川农业大学预防兽医研究所;四川普莱美生物科技有限公司国家实验猕猴种源基地;
【基金】：国家科技支撑计划项目(2014BAI03B01) 国家重大科学仪器设备开发专项(2013YQ49085906)
【分类号】：R587.1;R-332

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