石菖蒲挥发油有效成分联合左旋多巴对6-羟基多巴诱导帕金森病模型大鼠血脑屏障的影响

发布时间：2018-04-19 10:16

本文选题：石菖蒲挥发油 + 左旋多巴　；参考：《时珍国医国药》2017年01期

【摘要】：目的研究石菖蒲挥发油有效成分联合左旋多巴对6-羟基多巴诱导帕金森病模型大鼠血脑屏障的影响。方法定位于SPF级SD大鼠左脑的内侧前脑束(前囟后:-4.4 mm,中线旁:-1.2 mm,硬膜下:-7.8 mm),注射24μg的6-羟基多巴,造模1个月后,将造模大鼠腹腔注射0.5 mg/kg的阿扑吗啡,大鼠向健侧旋转数大于7圈/min为成功PD模型。将40只PD模型大鼠随机分为4组,每组10只,雌雄各半,即模型组,左旋多巴组(60 mg/kg),β-细辛醚组(15 mg/kg),联合用药组(左旋多巴60 mg/kg+β-细辛醚15 mg/kg)。给药组按上述剂量灌胃给药,一天2次,给药30天。末次给药1 h后,冰上迅速分离皮质。高效液相色谱法(HPLC)检测左旋多巴和多巴胺含量;流式细胞术检测皮质紧密连接蛋白(Zonula occludens 1,ZO-1)、紧密连接相关蛋白(Occludin)和肌动蛋白(Actin)含量;免疫组化检测紧密连接相关蛋白5(Claudin-5)。结果与假手术组比较,模型组大鼠的左旋多巴和多巴胺含量都显著减少(P0.05),ZO-1、Occludin、Actin和Claudin-5的表达无显著变化(P0.05);与模型组比较,联合用药组大鼠的左旋多巴和多巴胺含量都显著增加(P0.01),ZO-1、Occludin、Actin和Claudin-5的表达都显著减少(P0.05);与左旋多巴组比较,联合用药组大鼠的左旋多巴和多巴胺含量显著增加(P0.05),联合用药组大鼠的ZO-1、Occludin、Actin和Claudin-5的表达都显著减少(P0.05)。结论石菖蒲挥发油有效成分β-细辛醚联合左旋多巴具有一定提高PD模型大鼠皮质中BBB通透性的作用。
[Abstract]:Objective to study the effect of volatile oil of Acorus calamus combined with levodopa on the blood-brain barrier induced by 6-hydroxydopa in Parkinson's disease model rats.Methods the medial forebrain tract of the left brain of SPF SD rats (at the anterior fontanelle: -4.4 mm, at the midline: -1.2 mm, subdural at 1: -7.8 mm) was injected with 24 渭 g 6-hydroxydopa. After one month, the rats were injected intraperitoneally with apomorphine of 0.5 mg/kg.The successful PD model was established in rats with healthy side rotation number greater than 7 laps / min.Forty PD model rats were randomly divided into 4 groups, 10 rats in each group, male and female half, model group, levodopa group, 尾 -asarone group, 15 mg / kg group and 15 mg / kg group (levodopa 60 mg/kg 尾 -asarone 15 mg / kg).The drug group was given orally at the above dosage, twice a day for 30 days.One hour after the last administration, the cortex was rapidly separated from the ice.High performance liquid chromatography (HPLC) was used to detect the contents of levodopa and dopamine; flow cytometry was used to detect the content of cortical tight junction protein Zonula occludens 1 (ZO-1) and actin (Actinin).Results compared with the sham-operated group, the contents of levodopa and dopamine in the model group decreased significantly the expression of actin and Claudin-5 of ZO-1 Occludinia in the model group, while those in the model group were similar to those in the model group.The contents of levodopa and dopamine in combination group increased significantly the expression of P0.01ZO-1Occludinia actin and Claudin-5, which were significantly lower than those in levodopa group.The contents of levodopa and dopamine increased significantly in combination group, and the expression of ZO-1 Occludinin actin and Claudin-5 decreased significantly in combination group.Conclusion 尾 -asarone combined with levodopa can improve the permeability of BBB in the cortex of PD model rats.
【作者单位】：海南医学院;岭南师范学院;中山大学附属第六医院药学部;湛江市第二人民医院;广东省中医院(广东省中医药科学院广州中医药大学第二临床医学院);
【基金】：海南省自然基金项目(No.20168266) 海南省科协青年科技人才学术创新计划项目(No.HAST201635) 海南医学院科研培育基金项目(No.HY2015-01) 2015年学校创新计划——优秀博士论文培养项目(No.A1-AFD018161Z01024)
【分类号】：R285.5;R-332

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