越南起源食蟹猴MHCⅠ类等位基因多态性研究

发布时间：2018-04-20 04:41

本文选题：食蟹猴 + MHC-I等位基因　；参考：《华南理工大学》2011年硕士论文

【摘要】：食蟹猴（Macaca fascicularis），作为常用的一种实验动物，目前广泛用于包括猴免疫缺陷病毒(Simian immunodeficiency virus)感染和器官移植研究在内的生物医药研究。由于1978年印度颁布了恒河猴出口禁令，以及现有科学研究对实验猴需求量的快速增长，食蟹猴已逐渐成为一种潜在的理想实验猴。现有研究表明，清楚的MHC（major histocompatibility complex）基因多态性背景，对于实验动物模型在病理研究中的应用具有重要的意义。动物MHC遗传背景不清晰是限制我们在重大疾病研究中取得突破性进展的无法逾越的障碍之一。所以其MHC基因多态性研究已变得十分紧迫。目前，国际非人灵长类动物MHC命名委员会（IPD）已命名Mafa-A等位基因299个（包括Mafa-A1、A2、A3、A4、A5、A6等位基因），Mafa-B等位基因294个。除此之外，有关食蟹猴MHC多态性的研究报道较少。鉴于此，本研究以华南灵长类动物研究中心饲养的健康越南起源食蟹猴为研究对象，通过提取总RNA与RT-PCR，制备合成第一链cDNA，，并设计通用引物在cDNA池中筛选MHC-I类等位基因和对30个食蟹猴个体进行基因型分型。结果如下：共鉴定Mafa-A等位基因54个，其中有28个等位基因尚未报道，为新的等位基因；共鉴定到Mafa-B等位基因65个，其中有23个等位基因尚未报道，为新的等位基因。同时，本研究还阐明了食蟹猴MHC-I类等位基因在群体中的分布规律：每只实验猴有1-4个Mafa-A等位基因表达，有1-7个Mafa-B等位基因表达，与文献报道相符。而且本文还鉴定到一些高频等位基因：Mafa-B*007:01:01、Mafa-A1*022:06、Mafa-B*002:04、Mafa-B*039:01、Mafa-B*085:01、Mafa-A1*081:01:01和Mafa-A3*13:07，它们在群体中表达的频率分别为24.24%、19.44%、18.18%、18.18%、18.18%、16.67%、16.67%。本研究尚未鉴定到Mafa-A与Mafa-B共表达的高频等基因。此外，还发现了部分MHC I类等位基因为越南起源食蟹猴与其他起源如毛里求斯起源、印度尼西亚起源食蟹猴所共有，这也为阐述食蟹猴的演化过程提供一定的科学依据。
[Abstract]:As a commonly used experimental animal, Macaca fascicularis is widely used in biomedical research, including the infection of Simian immunodeficiency virus and organ transplantation. Due to the ban on the export of rhesus monkeys issued by India in 1978 and the rapid growth in demand for experimental monkeys by existing scientific research, crab-eating monkeys have gradually become a potential ideal experimental monkey. Current studies have shown that a clear background of MHC(major histocompatibility complex gene polymorphism is of great significance for the application of experimental animal models in pathological studies. The unclear genetic background of animal MHC is one of the insurmountable obstacles that limit our breakthrough in the research of major diseases. Therefore, the study of MHC gene polymorphism has become very urgent. At present, the International MHC Nomenclature Committee of nonhuman primates has named 299 Mafa-A alleles, including 294 Mafa-B alleles. In addition, there are few studies on MHC polymorphism in crab-eating monkeys. In view of this, a healthy Vietnamese origin crab-eating monkey raised by South China primate Research Center was used as the research object. The first strand cDNA was synthesized by extracting total RNA and RT-PCR. Universal primers were designed to screen MHC-I alleles in cDNA pool and genotyping of 30 individuals. The results were as follows: a total of 54 Mafa-A alleles were identified, of which 28 alleles were not reported as new alleles, and 65 Mafa-B alleles were identified, of which 23 alleles were not reported as new alleles. At the same time, the distribution of MHC-I alleles in the population was elucidated: each experimental monkey had 1-4 Mafa-A alleles and 1-7 Mafa-B alleles, which was consistent with the literature reports. In addition, some high frequency alleles Mafa-Bn00711011 Mafa-A1: 02206 Mafa-B: 039041: 03901Mafa-Bn08508501Mafa-A 008101W and Mafa-A3: 1307 have been identified. The frequency of their expression in the population is 24.24419.4418.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.1818.18@@ This study has not identified the Mafa-A and Mafa-B coexpression of high frequency genes. In addition, some MHC class I alleles were found to be shared by Vietnamese origin crab-eating monkeys and other origins such as Mauritius, Indonesia origin crab-eating monkeys, which provides a scientific basis for explaining the evolutionary process of crab-eating monkeys.
【学位授予单位】：华南理工大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332

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