单颗粒示踪技术及其在病毒侵染机制研究中的应用

发布时间：2018-04-20 03:12

本文选题：病毒 + 单颗粒示踪技术　；参考：《病毒学报》2017年04期

【摘要】：病毒侵染宿主细胞是一个复杂的动态过程,且涉及病毒与细胞多种成分的相互作用,病毒侵染机制研究对研制抗病毒药物以及病毒性疾病的预防和治疗至关重要。单颗粒示踪技术是可视化研究生物动态过程的重要工具,能够研究病毒在活细胞中的侵染行为,现已成为病毒侵染机制研究的有效手段。此文综述了单颗粒示踪技术中病毒标记物、标记方法、活细胞成像及分析进展,以流行性感冒病毒和人免疫缺陷病毒为例阐述了该技术在病毒侵染机制研究中的应用,对该技术的应用前景进行了展望。此文旨在为病毒侵染机制研究介绍新的技术手段、推动病毒学研究。
[Abstract]:Virus infection of host cells is a complex dynamic process and involves the interaction between virus and various components of cells. The study of virus infection mechanism is very important for the development of antiviral drugs and the prevention and treatment of viral diseases. Single particle tracer technology is an important tool to visualize the biological dynamic process and can study the infection behavior of virus in living cells. It has become an effective means to study the mechanism of virus infection. In this paper, the advances of virus markers, labeling methods, living cell imaging and analysis in single particle tracer technique are reviewed. The application of this technique in the study of virus infection mechanism is described with the examples of influenza virus and human immunodeficiency virus. The application prospect of this technology is prospected. The purpose of this paper is to introduce new techniques for the study of virus infection mechanism and to promote virology research.
【作者单位】：河南农业大学生命科学学院;
【基金】：国家自然科学基金(项目号:31490600),题目:猪繁殖与呼吸综合征病毒的致病机理研究~~
【分类号】：R373

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