多烯磷脂酰胆碱对树突状细胞的抑制作用研究

发布时间：2018-04-20 20:29

  本文选题：多烯磷脂酰胆碱 + 树突状细胞　； 参考：《中国现代药物应用》2016年24期

【摘要】：目的研究多烯磷脂酰胆碱(PPC)对树突状细胞(DC)表型分化及分泌炎症因子的影响,探讨PPC抗炎作用。方法 8只SPF纹雌性Balb/c小鼠,随机分为四组,每组2只。运用免疫磁珠分析小鼠脾脏DC,分别加入PPC(PPC组,2只)、脂多糖(LPS,LPS组,2只)、PPC+LPS(PPC+LPS组,2只)以及磷酸盐缓冲液(PBS,PBS组,2只)进行刺激。24 h后收集细胞及培养上清,流式细胞术检测DC表面活化分子表达情况,流式微珠阵列法(CBA)检测细胞因子分泌情况。结果 PPC组DC表面CD40、CD80、CD86、主要组织相容性复合体(MHC)-Ⅱ分别为(23.43±2.79)%、(19.41±1.33)%、(44.30±3.16)%、(80.87±1.32)%,低于PBS组的(29.10±8.14)%、(31.81±1.02)%、(47.70±6.32)%、(83.17±2.84)%;且显著低于LPS组的(42.33±0.58)%、(63.57±2.61)%、(56.07±0.06)%、(86.20±0.95)%(P0.01);PPC+LPS组CD40、CD80、CD86、MHC-Ⅱ分别为(16.73±3.14)%、(16.12±1.92)%、(43.60±0.95)%、(78.83±1.46)%,显著低于LPS组(P0.01)。PPC组和PPC+LPS组白细胞介素(IL)-6、肿瘤坏死因子(TNF)显著低于LPS组(P0.01)。PPC+LPS组单核细胞趋化蛋白-1(MCP-1)水平明显低于LPS组(P0.01)。结论 PPC可通过抑制DC活化及炎症因子释放,发挥抗炎效果。
[Abstract]:Objective to investigate the effect of polyenylphosphatidylcholine (PPC) on phenotype differentiation and secretion of inflammatory cytokines in dendritic cells (DC). Methods eight SPF striated female Balb/c mice were randomly divided into four groups with 2 mice in each group. The spleen of mice was analyzed by immunomagnetic beads. The cells were collected and cultured in PPC(PPC group (n = 2), lipopolysaccharide (LPS) group (n = 2) and PPC LPS(PPC LPS group (n = 2) and phosphate buffer solution (PBS) group (n = 2). Flow cytometry was used to detect the expression of activated molecules on DC surface and CBA was used to detect cytokine secretion. 缁撴灉 PPC缁凞C琛ㄩ潰CD40,CD80,CD86,涓昏�佺粍缁囩浉瀹规�у�嶅悎浣�(MHC)-鈪″垎鍒�涓�(23.43卤2.79)%,(19.41卤1.33)%,(44.30卤3.16)%,(80.87卤1.32)%,浣庝簬PBS缁勭殑(29.10卤8.14)%,(31.81卤1.02)%,(47.70卤6.32)%,(83.17卤2.84)%;涓旀樉钁椾綆浜嶭PS缁勭殑(42.33卤0.58)%,(63.57卤2.61)%,(56.07卤0.06)%,(86.20卤0.95)%(P0.01);PPC LPS缁凜D40,CD80,CD86,MHC-鈪″垎鍒�涓�(16.73卤3.14)%,(16.12卤1.92)%,(43.60卤0.95)%,(78.83卤1.46)%,鏄捐憲浣庝簬LPS缁，

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