瘙痒介质在小鼠变应性接触性皮炎模型背根神经节中的表达

发布时间：2018-04-20 20:45

本文选题：CBL/小鼠 + 变应性接触性皮炎　；参考：《中国皮肤性病学杂志》2017年07期

【摘要】：目的建立半抗原2,4-二硝基氟苯(DNFB)诱发C57BL/6小鼠变应性接触性皮炎(ACD)模型,检测瘙痒介质胃泌素释放肽(GRP),P物质和TRPV-1分子在背根神经节(DRG)中的表达并探讨它们对在ACD伴发的慢性瘙痒的作用。方法外用DNFB建立C57BL/6小鼠的ACD模型,观察皮炎症状和皮肤病理等指标,检测血清总IgE和IL-4浓度,并分析GRP,P物质和TRPV-1在颈部背根神经节(DRG)的表达水平变化。结果与对照组相比,DNFB模型组小鼠颈背部皮肤表现出明显炎症性皮损,病理提示表皮增厚、真皮肥大细胞浸润,血清IgE和IL-4明显升高,GRP,P物质和TRPV-1的mRNA在颈部DRG表达升高。结论外用半抗原DNFB成功建立了C57BL/6小鼠ACD模型,GRP,P物质和TRPV-1在ACD模型瘙痒中有重要作用。
[Abstract]:Objective to establish an ACD-induced allergic contact dermatitis (ACD) model in C57BL/6 mice induced by hapten 2N 4-dinitrofluorobenzene (DNFB). To detect the expression of pruritus mediators gastrin releasing peptide (gastrin releasing peptide) and TRPV-1 in DRG and to explore their effects on chronic pruritus associated with ACD. Methods the ACD model of C57BL/6 mice was established by DNFB. The symptoms of dermatitis and skin pathology were observed. The serum total IgE and IL-4 levels were measured. The expression of GRPP-P and TRPV-1 in the cervical dorsal root ganglion (DRG) was analyzed. Results compared with the control group, the skin of the mice in the DNFB group showed obvious inflammatory lesions. The pathological results showed that the epidermis thickened, the dermis mast cells infiltrated, and the serum IgE and IL-4 increased significantly the expression of GRP P and mRNA of TRPV-1 in the neck DRG. Conclusion the ACD model of C57BL/6 mice was successfully established by external use of hapten DNFB and TRPV-1 and GRP P played an important role in the pruritus of ACD model.
【作者单位】：广州医科大学附属第二医院广东省过敏反应与免疫重点实验室/呼吸疾病国家重点实验室变态反应研究室;
【分类号】：R-332;R758.2

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