特异性调控前额叶皮质锥体细胞活性对大鼠内脏痛行为的影响

发布时间：2018-04-21 23:33

本文选题：内侧前额叶皮质 + 锥体细胞　；参考：《神经解剖学杂志》2017年05期

【摘要】：目的:通过光遗传学手段,特异性调控内侧前额叶皮质(medial prefrontal cortex,m PFC)锥体细胞活性的变化,观察对于大鼠内脏痛及焦虑行为的影响。方法:雄性成年大鼠分为3组,随机注入AAV_(2/9)-Ca MKII-mcherry,AAV_(2/9)-Ca MKII-ChR2-mcherry以及AAV_(2/9)-Ca MKII-Arch T-mcherry病毒,特异性感染锥体细胞。病毒注射4周后,将光纤埋入双侧m PFC内,之后通过给予蓝光或黄光刺激激活含ChR2或Arch T的神经元,观察对于大鼠内脏痛和焦虑行为的影响。结果:AAV注射很好地感染了m PFC内的锥体细胞,表达ChR2的大鼠经蓝光照射后Fos表达量增高,内脏痛反应和焦虑行为受到抑制。表达Arch T的大鼠经黄光刺激后Fos表达量降低,内脏痛反应和焦虑行为增加。结论:兴奋m PFC内的锥体细胞对于内脏痛和焦虑产生明显抑制,而抑制锥体细胞则增强内脏痛和焦虑行为。
[Abstract]:Aim: to specifically regulate the pyramidal cell activity of medial prefrontal cortexm in medial prefrontal cortex by means of photogenetics, and observe the effects on visceral pain and anxiety behavior in rats. Methods: male adult rats were randomly divided into 3 groups and infected with AAV_(2/9)-Ca MKII-mcherrytir AAVI 2 / 9 MKII-ChR2-mcherry and AAV_(2/9)-Ca MKII-Arch T-mcherry virus at random. After 4 weeks of virus injection, the optical fibers were embedded into bilateral m PFC, then the neurons containing ChR2 or Arch T were activated by blue or yellow light stimulation to observe the effects on visceral pain and anxiety behavior in rats. Results the pyramidal cells in m PFC were well infected with the injection of 1% AAV. The expression of Fos was increased and the visceral pain response and anxiety behavior were inhibited after ChR2 was irradiated by blue light in rats. The expression of Fos decreased, visceral pain response and anxiety behavior increased after yellow light stimulation in rats expressing Arch T. Conclusion: the pyramidal cells in excitatory m PFC significantly inhibit visceral pain and anxiety, while inhibition of pyramidal cells enhances visceral pain and anxiety behavior.
【作者单位】：西安医学院公共卫生系;西安交通大学基础医学院生理学与病理生理学系;
【基金】：陕西省自然科学基础研究计划-青年项目(2017JQ8058) 陕西省教育厅科研计划项目(17JK0659,15JK1628) 陕西省大学生创新创业训练计划平台(201711840024) 西安医学院博士科研启动基金项目(2016DOC10);西安医学院大学生创新基金项目(2016DXS1-22) 国家自然科学基金青年项目(81300972)
【分类号】：R338

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