肝素结合表皮生长因子对肝移植模型大鼠肝脏炎症因子及细胞再生的影响

发布时间：2018-04-22 06:21

本文选题：肝移植 + 肝细胞生长因子　；参考：《胃肠病学和肝病学杂志》2016年12期

【摘要】：目的研究肝素结合表皮生长因子(heparin-binding epidermal growth factor-like growth factor,HB-EGF)对肝移植模型大鼠肝脏炎症因子及细胞再生的影响。方法选择60只SD雄性大鼠并建立肝移植动物模型,随机分为A、B、C三组,B组皮下注入0.1mg/kg肝细胞生长因子,C组皮下注射0.1 mg/kg HB-EGF,A组给予相等剂量生理盐水进行干预。干预后第1、3、5、7天时,比较三组肝脏湿重、肝功能、炎性因子含量。结果干预后第1、3、5、7天时,B组、C组大鼠移植肝脏的湿重均明显高于A组(P0.05),血清丙氨酸氨基转氨酶(ALT)、总胆红素(TBIL)含量均明显低于A组(P0.05);干预后第3、5、7天时,B组、C组血清白蛋白(ALB)含量明显高于A组(P0.05);干预后第1、3、5、7天时,B组、C组大鼠肝脏组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量均明显低于A组(P0.05);C组大鼠肝脏组织中TNF-α、IL-6含量明显低于B组(P0.05)。结论 HB-EGF能促进肝移植模型大鼠肝细胞再生,其作用机制可能与保护肝功能、减轻炎症反应有关。
[Abstract]:Objective to study the effect of heparin-binding epidermal growth factor-like growth factor-HB-EGFon on inflammatory cytokines and cell regeneration in liver transplantation rats. Methods 60 Sprague-Dawley male rats were selected and established the liver transplantation model. The rats were randomly divided into three groups: group B: 0.1mg/kg hepatocyte growth factor group C was subcutaneously injected with 0.1 mg/kg HB-EGFGF-A group and treated with the same dose of normal saline. The wet weight of liver, liver function and the content of inflammatory factors in the three groups were compared on the 7th day after intervention. Results the wet weight of liver grafts in group B was significantly higher than that in group A (P 0.05), and the levels of serum alanine aminotransferase (alt) and total bilirubin (TBIL) in group B were significantly lower than those in group A (P 0.05) on the 1st day after intervention, and in group B at 357 days after intervention, the serum levels of serum alanine aminotransferase (alt) and total bilirubin (TBIL) in group C were significantly lower than those in group A (P 0.05). The contents of TNF- 伪 -TNF- 伪 TNF- 伪 and IL-6 in liver tissue of rats in group B were significantly lower than those in group A (P 0.05), and the contents of TNF- 伪 IL-6 in group C were significantly lower than those in group B at 7 days after intervention. Conclusion HB-EGF can promote the regeneration of hepatocytes in rat model of liver transplantation, and its mechanism may be related to the protection of liver function and the alleviation of inflammatory reaction.
【作者单位】：厦门市第二医院肝胆外科;
【基金】：厦门市科技计划指导性项目(3502Z20139012)
【分类号】：R657.3;R-332

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