结核分枝杆菌clpP2抗原表位预测与分析

发布时间：2018-04-23 06:34

本文选题：结核分枝杆菌 + clpP　；参考：《四川大学学报(医学版)》2017年02期

【摘要】：目的预测结核分枝杆菌酪蛋白酶蛋白水解亚基单位2(clpP2)的抗原表位,为结核病疫苗、药物研制等提供新的靶点。方法以clpP2蛋白的氨基酸序列为基础,采用生物信息软件DNA Star预测二级结构;运用SWISS-MODEL在线软件进行同源建模;运用VaxiPred软件综合预测T细胞抗原表位,用ABCpred、COBEPro和BepiPredPred软件综合预测其B细胞抗原表位;采用DNA Star Protean、SignalP、TMHMM在线软件和NCBI数据库分析其免疫学相关信息。结果 clpP2蛋白结构丰富,含有较多潜在细胞毒性T细胞和辅助T细胞表位;也含有较多的B细胞线性和构象优势表位,这些区域亲水性、表面可能性和柔韧性指数都较高。结论 clpP2蛋白具有诱导免疫应答的潜能,可以作为结核监测、治疗、预防的新靶点。
[Abstract]:Objective to predict the epitopes of casein proteolytic subunit 2clpP2of Mycobacterium tuberculosis, and to provide a new target for tuberculosis vaccine and drug development. Methods based on the amino acid sequence of clpP2 protein, the secondary structure was predicted by the bioinformatics software DNA Star, the homology model was established by SWISS-MODEL online software, and the T cell epitope was comprehensively predicted by VaxiPred software. The B cell epitopes were comprehensively predicted by ABCpred software, and the immunological information of B cell epitopes was analyzed by DNA Star protein SignalPherm online software and NCBI database. Results clpP2 protein was rich in structure, containing more potential cytotoxic T cells and auxiliary T cell epitopes, and also containing more B cell linear and conformational dominant epitopes, which had high hydrophilicity, surface possibility and flexibility index. Conclusion clpP2 protein has the potential to induce immune response and can be used as a new target for tuberculosis surveillance, treatment and prevention.
【作者单位】：四川大学华西公共卫生学院卫生检验与检疫系;
【基金】：国家自然科学基金面上项目(No.31570924) 四川省科技厅国际合作项目(No.2017HH0080)资助
【分类号】：R378.911

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